中药药理与临床2026，Vol.42Issue(5)：91-99,9.
益母草醇提物调控Rho A/ROCK1信号通路对血管平滑肌细胞表型转化的影响
Effects of Alcohol Extracts of Leonuri Herba on Phenotypic Transformation of Vascular Smooth Muscle Cells via RhoA/ROCK1 Signaling Pathway
摘要
Abstract
Objective:To investigate the effects of alcohol extracts of Leonuri Herba on the proliferation,migration,and phenotypic transformation of vascular smooth muscle cells(VSMCs)induced by platelet-derived growth factor-BB(PDGF-BB),and its mechanism based on the Ras homolog gene-Rho-related coiled-coil kinase(RhoA/ROCK)signa-ling pathway.Methods:A primary rat VSMC model induced by PDGF-BB was established.The CCK-8 assay was used to determine the safe dose of alcohol extracts of Leonuri Herba for VSMCs.The BrdU assay was used to assess the effect of alcohol extracts of Leonuri Herba on VSMC proliferation.Cell migration and invasion abilities were detected by scratch and Transwell assays.Flow cytometry combined with PI staining was employed to detect the effect of alcohol extracts of Leonuri Herba on the cycle distribution of VSMCs.Immunofluorescence staining was used to investigate the effect of al-cohol extracts of Leonuri Herba on cytoskeletal remodeling in VSMCs.Western blot was utilized to detect the phenotypic transformation of VSMCs from contractile to synthetic phenotype markers,as well as proteins related to the RhoA/ROCK signaling pathway.Results:Compared with the blank control group,VSMCs in the model control group exhibited en-hanced migration ability(P<0.01)and significant S-phase arrest(P<0.01).The expression of contractile phenotype markers calponin and SM22α was significantly downregulated(P<0.05),while the phosphorylation levels of synthetic phenotype markers OPN,PCNA,RHOA,ROCK,and JNK were significantly upregulated(P<0.05 or P<0.01).Com-pared with the model control group,alcohol extracts of Leonuri Herba significantly inhibited PDGF-BB-induced VSMC proliferation,migration,and phenotypic transformation(P<0.01).Treatment with alcohol extracts of Leonuri Herba at 25,50,and 100 μg/mL significantly upregulated the expression of calponin,SM22α,and α-SMA(P<0.05 or P<0.01)and downregulated the phosphorylation levels of OPN,PCNA,RHOA,ROCK1,and JNK(P<0.05 or P<0.01).Conclu-sion:Alcohol extracts of Leonuri Herba may improve vascular remodeling by inhibiting the proliferation,migration,and transformation of primary rat VSMCs to the synthetic phenotype induced by PDGF-BB,through regulation of the RhoA/ROCK1 signaling pathway and suppression of JNK phosphorylation.关键词
益母草/血管平滑肌细胞/增殖/迁移/表型转化Key words
Leonuri Herba/Vascular smooth muscle cells/Proliferation/Migration/Phenotypic transformation引用本文复制引用
宋柯妍,刘金杰,李梓宁,冉怡,张晗,李霄..益母草醇提物调控Rho A/ROCK1信号通路对血管平滑肌细胞表型转化的影响[J].中药药理与临床,2026,42(5):91-99,9.基金项目
天津市科技计划项目(编号:24ZYJDSS00320) （编号:24ZYJDSS00320）
现代中药新质生产力科技创新工程专项(编号:24ZXZKSY00010) （编号:24ZXZKSY00010）
组分中药国家重点实验室资助课题(编号:CBQM2022106). （编号:CBQM2022106）
