中药药理与临床2026，Vol.42Issue(6)：59-66,8.
黄芪甲苷对辐射诱导的大鼠H9C2心肌细胞凋亡及miR-134-5p/BDNF/PI3K信号通路的影响
Effects of Astragaloside Ⅳ on Radiation-Induced Apoptosis of H9C2 Cardiomyocytes in Rats and miR-134-5p/BDNF/PI3K Signaling Pathway
摘要
Abstract
Objective:To explore the protective effects and related mechanisms of Astragaloside Ⅳ(ASⅣ)on ra-diation-induced cardiomyocyte injury and apoptosis.Methods:H9C2 cells were divided into a normal control group,a model control group(6 Gy X-ray irradiation),and an ASⅣ 6 μg/mL group(cells pretreated before irradiation).Cell viability was detected using the CCK-8 assay.Reactive oxygen species(ROS)levels were detected with DCFH-DA probes.Superoxide dismutase(SOD)activity and malondialdehyde(MDA)content were measured with commercial kits.Mitochondrial membrane potential was assessed by the JC-1 method.Apoptosis rate was detected by Annexin V-FITC/PI double staining flow cytometry and Hoechst 33342 staining.MiR-134-5p expression was determined by qRT-PCR.BDNF,PI3K,AKT,p-AKT,BCL-2,and BAX protein expression was analyzed by Western blot.Results:Compared with the normal control group,the model control group showed increased ROS levels and MDA content,decreased SOD activity,an increased percentage of mitochondrial membrane potential loss,significantly increased apoptosis rate,upregu-lation of miR-134-5p,downregulation of BDNF,PI3K,p-AKT,and BCL-2 protein expression,and upregulation of BAX expression(P<0.05).Compared with the model control group,the ASⅣ 6 μg/mL group showed reduced ROS levels and MDA content,increased SOD activity,a decreased percentage of mitochondrial membrane potential loss,reduced ap-optosis rate,downregulation of miR-134-5p,upregulation of BDNF,PI3K,p-AKT,and BCL-2 protein expression,and downregulation of BAX expression(P<0.05).Conclusion:ASⅣ can attenuate oxidative stress and mitochondrial dam-age induced by irradiation and reduce cardiomyocyte apoptosis.Its protective effect is associated with regulation of the miR-134-5p/BDNF/PI3K signaling pathway.关键词
黄芪甲苷/辐射损伤/心肌细胞/微小核糖核酸-134-5p/细胞凋亡Key words
Astragaloside Ⅳ/Radiation injury/Cardiomyocytes/MicroRNA-134-5p/Apoptosis引用本文复制引用
顾静,荆爽,韩晓斐,方丹,董晓丽,颉亚辉..黄芪甲苷对辐射诱导的大鼠H9C2心肌细胞凋亡及miR-134-5p/BDNF/PI3K信号通路的影响[J].中药药理与临床,2026,42(6):59-66,8.基金项目
国家自然科学基金(编号:82360878). （编号:82360878）
