组织工程与重建外科杂志2026，Vol.22Issue(3)：261-268,8.DOI:10.3969/j.issn.1673-0364.2026.03.005
负载脂肪间充质干细胞外泌体的HAMA/PEGDA/GelMA水凝胶促进全层皮肤创面修复的实验研究
HAMA/PEGDA/GelMA hydrogel loaded with adipose-derived mesenchymal stem cell exosomes promotes the repair of full-thickness skin wounds
摘要
Abstract
Objective To prepare HAMA/PEGDA/GelMA hydrogel materials loaded with adipose-derived mesenchymal stem cell exosomes(hADMSC-Exo)and explore their effect on the repair of full-thickness skin defects.Methods hADMSC-Exo were extracted and identified by electron microscopy,particle size,and Western Blot.Composite hydrogel materials were constructed by photo-crosslinking.Their microstructure was detected by electron microscopy,and the exosome release characteristics were observed by extraction method.The extract of composite hydrogels was co-cultured with human umbilical vein endothelial cells(hUVECs)to observe exosome uptake and cell migration.A mouse full-thickness skin defect model was established.On the 12th day after surgery,HE and Masson histological staining were used to evaluate the role in wound repair.Results The composite hydrogel materials were successfully prepared.hADMSC-Exo could be slowly released and taken up by hUVECs,and significantly promoted cell migration(P<0.05).Animal experiment results showed that on the 12th day,compared with the negative control group,the HAMA/PEGDA/GelMA+Exo group had a higher wound closure rate,better collagen deposition.Conclusion HAMA/PEGDA/GelMA composite hydrogels loaded with hADMSC-Exo can significantly accelerate the healing of full-thickness skin defects.The mechanism may be related to regulating the inflammatory response,promoting angiogenesis and collagen deposition,which provides experimental basis and a new strategy for the development of new wound coverage dressings.关键词
水凝胶/脂肪间充质干细胞/外泌体/创面修复Key words
Hydrogel/Adipose-derived mesenchymal stem cells/Exosomes/Wound repair分类
医药卫生引用本文复制引用
郭彩茹,王浩洁,马湘宇,牛宇杰,董荣启,李明鸣..负载脂肪间充质干细胞外泌体的HAMA/PEGDA/GelMA水凝胶促进全层皮肤创面修复的实验研究[J].组织工程与重建外科杂志,2026,22(3):261-268,8.基金项目
河南省医学科技攻关计划联合共建项目(LHGJ20230824) （LHGJ20230824）
洛阳市公益性行业医疗卫生专项(2302041Y). （2302041Y）
