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A practical guide for characterization of novel CRISPR-Cas systems with Pro-CRISPR factors

Ao Sun Shu-Lin Jin Jun-Jie Gogo Liu

Biophysics Reports2026，Vol.12Issue(2)：P.85-99,15.

Biophysics Reports2026，Vol.12Issue(2)：P.85-99,15.DOI:10.52601/bpr.2025.240066

A practical guide for characterization of novel CRISPR-Cas systems with Pro-CRISPR factors

Ao Sun ¹Shu-Lin Jin ¹Jun-Jie Gogo Liu¹

作者信息

1. Beijing Frontier Research Center for Biological Structure,Beijing Advanced Innovation Center for Structural Biology,State Key Laboratory of Membrane Biology,Tsinghua-Peking Center for Life Sciences,School of Life Sciences,Tsinghua University,Beijing 100084,China
折叠

摘要

关键词

CRISPR-Cas system/Genome editing/Cas nuclease/Pro-CRISPR/Protein purification/Biochemical properties

分类

生物科学

引用本文复制引用

Ao Sun,Shu-Lin Jin,Jun-Jie Gogo Liu..A practical guide for characterization of novel CRISPR-Cas systems with Pro-CRISPR factors[J].Biophysics Reports,2026,12(2):P.85-99,15.

基金项目

supported by The National Key R&D Program(2022YFF1002801 to JJ.G.Liu) （2022YFF1002801 to JJ.G.Liu）

Ministry of Agriculture and Rural Affairs of China(JJ.G.Liu) （JJ.G.Liu）

the New Cornerstone Science Foundation through the XPLORER PRIZE(JJ.G.Liu) （JJ.G.Liu）

The STI2030-Major Projects(2022ZD0213900 to JJ.G.Liu) （2022ZD0213900 to JJ.G.Liu）

the National Natural Science Foundation of China(32150018 to JJ.G.Liu and 323B2048 to A.Sun)。（32150018 to JJ.G.Liu and 323B2048 to A.Sun）

Biophysics Reports

ISSN：2364-3439

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