广东医学2026，Vol.47Issue(6)：845-851,7.DOI:10.13820/j.cnki.gdyx.20254608
免疫纳米磁珠分离孕妇外周血中胎儿有核红细胞的有效性研究
Efficacy of immunomagnetic nanobead-based isolation of fetal nucleated red blood cells from maternal peripher-al blood
摘要
Abstract
Objective To evaluate the efficacy of isolating fetal nucleated red blood cells(FNRBCs)from mater-nal peripheral blood using antibody-labeled immunomagnetic nanobeads.Methods Fetal nucleated red blood cells were isolated from umbilical cord blood using density gradient centrifugation combined with immunomagnetic nanobead sep-aration.The purity of target cells was assessed by flow cytometry and further verified by immunofluorescence staining.Pe-ripheral blood samples from pregnant women and healthy non-pregnant controls were processed and analyzed using immu-nofluorescence.Simulated samples were prepared by mixing umbilical cord blood from male fetuses with peripheral blood from non-pregnant women.In addition,maternal peripheral blood samples from pregnancies with chromosomally normal fetuses and fetuses with aneuploidy were analyzed.Fluorescence in situ hybridization(FISH)was performed on isolated cells to evaluate chromosomal characteristics.Results Flow cytometry revealed that the proportions of double-positive cells for specific antibody fluorescence were(90.16±5.77)％in the positive fraction and(7.12±4.45)％in the nega-tive fraction.Triple co-expression of antibody signals was observed in positive cells but not in negative cells.Cells isola-ted from maternal peripheral blood of pregnant women showed co-expression of all three antibody markers,whereas no such expression was observed in the control group.FISH analysis detected Y chromosomes in all simulated samples.A-mong five maternal peripheral blood samples from pregnancies with chromosomally normal fetuses,Y chromosomes were detected in two cases.Among four samples from pregnancies with confirmed fetal aneuploidy,two cases of trisomy 21,one case of trisomy 13,and one case of trisomy 18 were identified,consistent with amniotic fluid karyotype results.Conclu-sion Immunomagnetic nanobeads can effectively isolate fetal nucleated red blood cells from maternal peripheral blood and may serve as a promising approach for noninvasive detection of fetal aneuploidy.关键词
免疫标记纳米磁珠/胎儿有核红细胞/无创性产前诊断/出生缺陷防控Key words
immunomagnetic nanobeads/fetal nucleated red blood cells/noninvasive prenatal diagnosis/birth defect prevention分类
医药卫生引用本文复制引用
杨兴坤,周雅思,李炜辉,杜佳月,陈玥,夏承来..免疫纳米磁珠分离孕妇外周血中胎儿有核红细胞的有效性研究[J].广东医学,2026,47(6):845-851,7.基金项目
国家自然科学基金面上项目(82171713) （82171713）
