贵州农业科学2026，Vol.54Issue(7)：19-26,8.DOI:10.3969/j.issn.1001-3601.2026.07.004
基于BSA-seq技术的饲料油菜植株粗蛋白含量QTL定位研究
QTL Mapping of Crude Protein Content in Forage Rape Plants Based on BSA-seq Technology
摘要
Abstract
[Objective]The genetic loci and key genes regulating crude protein trait of forage rapeseed plants were analyzed to provide a reference for improvement of crude protein trait of forage rapeseed plants and breeding of high-quality forage rapeseed varieties.[Method]The BSA-seq technology was used to sequence and conduct association analysis on DNA mixed pool samples of parental lines 21SL-239 and 21SL-756 with extreme crude protein differences and F2 segregating population with extreme differences.[Result]A total of 37 regions significantly associated with crude protein content trait were identified within the 50.46-53.02 Mb interval on chromosome C08 of rapeseed.38 functional genes(ZS11C08G057720,ZS11C08G059050,ZS11C08G058760,etc.)related to protein synthesis,amino acid supply,protein degradation,97 functional genes(ZS11C08G058820,ZS11C08G057600,ZS11C08G061500,ZS11C08G061580,ZS11C08G061590,etc.)related to carbohydrate transport and metabolism,energy production and conversion,transcription regulation,etc.and 104 unknown function genes(ZS11C08G058790,ZS11C08G061450,ZS11C08G059590,etc.)were screened by a functional annotation technology.[Conclusion]One QTL locus related to crude protein content of forage rapeseed plants is located,and 239 candidate genes potentially related to crude protein content are screened.关键词
饲料油菜/粗蛋白/BSA-seq/QTL/高通量测序/候选基因Key words
forage rape/crude protein/BSA-seq/QTL/high-throughput sequencing/candidate gene分类
农业科技引用本文复制引用
吴宇瑶,钟伟,张云怡,王涛,张林,陈建军,王婧泽,代文东..基于BSA-seq技术的饲料油菜植株粗蛋白含量QTL定位研究[J].贵州农业科学,2026,54(7):19-26,8.基金项目
贵州省科技计划项目"饲料油菜植株粗蛋白含量的遗传及QTL定位研究"(黔科合基础-ZK[2022]一般289),"饲料油菜混合发酵提升菜籽粕利用价值的关键技术研究"(黔科合支撑[2023]一般073) （黔科合基础-ZK[2022]一般289）
中国烟草总公司贵州省科技项目"油菜茬烤烟-大豆带状复合种植模式创建与应用研究"(中烟黔科[2022]13号) （中烟黔科[2022]13号）
