贵州农业科学2026，Vol.54Issue(7)：78-87,10.DOI:10.3969/j.issn.1001-3601.2026.07.010
辣椒主要病害广谱生防木霉菌的筛选与评价及鉴定
Screening,Evaluation and Identification of Trichoderma,a Broad-spectrum Biocontrol Fungus,Against Major Diseases in Chili Pepper
摘要
Abstract
[Objective]The Trichoderma strains with a broad-spectrum biocontrol effect against 4 major diseases of Fusarium oxysporum,Phytophthora capsici,Athelia rolfsii and Colletotrichum capsici in chili pepper were isolated and screened to provide biocontrol resources for sustainable prevention and control of major diseases in chili peppers.[Method]The Trichoderma strains with an optimal antagonistic effect against 4 common diseases were isolated and screened from the rhizosphere soil of chili peppers at different growth stages by the gradient dilution method and plate confrontation method.The inhibition activity of the fermentation liquid of Trichoderma strains was determined by the colony diameter method.Meanwhile,the biocontrol effect was evaluated by the pot and in vitro pepper prevention and control experiments.The screened strains were identified in morphology and molecular biology.[Result]10 Trichoderma strains were isolated from the soil samples.The inhibitory rate of strain YDMM-07 against F.oxysporum,A.rolfsii,P.capsici and C.capsici was 83.71%,81.79%,93.24%and 85.51%respectively.The inhibitory rate of strain YDMM-07 sterile fermentation broth against F.oxysporum,A.rolfsii,P.capsici and C.capsici was 49.63%,31.82%,58.30%and 63.22%,separately.The prevention and control rate of strain YDMM-07 fermentation broth containing mycelia against F.oxysporum,P.capsici and A.rolfsii was 66.98%,71.00%and 62.98%in the pot experiment respectively,and the control rate of the sterile fermentation broth against C.capsici of in vitro pepper reached 52.39%.Strain YDMM-07 was identified as Trichoderma longibrachiatum according to its morphology and molecular biology.[Conclusion]T.longibrachiatum YDMM-07 strain with a good prevention and control effect against 4 major diseases of F.oxysporum,P.capsici,A.rolfsii and C.capsici can be used in development of antibiological agents against diseases in chili pepper.关键词
辣椒/常发性病害/生物防治/广谱拮抗/木霉菌/长枝木霉Key words
chili pepper/major diseases/biological control/broad-spectrum antagonism/Trichoderma/Longi-brachiatum分类
农业科技引用本文复制引用
卯婷婷,章行远,乔飞,班菲雪,刘少兰..辣椒主要病害广谱生防木霉菌的筛选与评价及鉴定[J].贵州农业科学,2026,54(7):78-87,10.基金项目
贵州省基础研究计划(自然科学)项目(黔科合基础-ZK[2023]一般191) （自然科学）
国家自然科学基金项目(32460710) （32460710）
贵州省重大科技计划项目(No.2025031) （No.2025031）
贵州省农业科学院关键核心技术项目(No.GJHX[2025]05) （No.GJHX[2025]05）
农药风险评估及有害生物靶向防治新兴人才团队项目(黔农科院新兴团队[2026]01号) （黔农科院新兴团队[2026]01号）
农业入侵物种绿色防控创新能力建设项目(黔科合服企[2023]011) （黔科合服企[2023]011）
贵州省农业微生物全省重点实验室平台项目(黔科合平台[2025]029) （黔科合平台[2025]029）
