海南医科大学学报2026，Vol.32Issue(13)：994-1003,10.DOI:10.13210/j.cnki.jhmu.20250708.004
加味薏苡附子败酱散对溃疡性结肠炎大鼠肠黏膜微循环障碍改善作用的研究
Effect of Modified Yiyi Fuzi Baijiang Powder on intestinal mucosal microcirculatory disturbance in rats with ulcerative colitis
摘要
Abstract
Objective:Explore the improvement effect of the Modified Yiyi Fuzi Baijiang Powder on intestinal mucosal micro-circulatory disturbance in rats with ulcerative colitis(UC)induced by dextran sulfate sodium(DSS).Methods:A total of 60 SPF-grade rats were randomly divided into a blank group,model group,mesalazine group,and low,medium,and high-dose Modified Yiyi Fuzi Baijiang Powder groups.Except for the blank group,rats in all other groups were treated with 3%DSS to establish the UC model.Following successful model establishment,rats in the mesalazine group and each dose of the traditional Chinese medi-cine group received corresponding treatments via gavage,while rats in both the blank and model groups were given an equivalent amount of physiological saline as a control.H&E staining was used to observe the pathological changes of colon tissue in each group.A blood rheology instrument was used to measure hematological parameters.ELISA was employed to detect serum levels of P-selectin,TXB2,and 6-Keto-PGF1α in the rats.Transmission electron microscopy was utilized to examine the ultrastructure of intestinal microvessels,while Masson's trichrome staining assessed the ratio of microvascular collagen fiber-positive area.Immu-nohistochemical methods were used to detect the levels of VEGF in colonic tissue and to measure MVD in the intestinal mucosa.Result:Compared to the blank group,the model group showed significant pathological damage to the colon,with significantly in-creased levels of blood rheological parameters,P-selectin,TXB2,VEGF,and MVD(P<0.05).The expression level of 6-Keto-PGF1α was significantly decreased(P<0.01),microvascular damage was significant,and the ratio of microvascular collagen fiber-positive area was significantly increased(P<0.01).Compared to the model group,each treatment group showed improvement in colonic pathological damage,with decreased levels of blood rheological parameters,P-selectin,TXB2,VEGF,and MVD(P<0.05).The expression level of 6-Keto-PGF1α was increased(P<0.01),microvascular damage was alleviated,and the ratio of mi-crovascular collagen fiber-positive area was significantly reduced(P<0.01).Among the traditional Chinese medicine groups,the high-dose group demonstrated the most significant efficacy.Conclusion:Modified Yiyi Fuzi Baijiang Powder can effectively im-prove intestinal mucosal microcirculatory disturbance in rats with UC.关键词
溃疡性结肠炎/加味薏苡附子败酱散/肠黏膜/微循环障碍Key words
Ulcerative colitis/Modified Yiyi Fuzi Baijiang Powder/Intestinal mucosa/Microcirculatory disturbance分类
医药卫生引用本文复制引用
何志成,陈江,李文晓,张露蓉,梁国强,江星星,韦永娜,周琴..加味薏苡附子败酱散对溃疡性结肠炎大鼠肠黏膜微循环障碍改善作用的研究[J].海南医科大学学报,2026,32(13):994-1003,10.基金项目
This study was supported by the Climbing Plan Project of Jiangsu Society of Traditional Chinese Medicine(PDJH2024035) （PDJH2024035）
Open Project of Jiangsu Academy of Traditional Chinese Medicine Schools(JSZYLP2024003) （JSZYLP2024003）
Suzhou Applied Basic Research(Medical and Health)Technology Innovation Project(SYWD2024012) （Medical and Health）
Suzhou Science and Technology Development Plan(Basic Research Medical Application Basic Research)Project(SKYD2023040). 江苏省中医药学会攀登计划项目(PDJH2024035) （Basic Research Medical Application Basic Research）
江苏省中医流派研究院开放课题(JSZYLP2024003) （JSZYLP2024003）
苏州市应用基础研究(医疗卫生)科技创新项目(SYWD2024012) （医疗卫生）
苏州市科技发展计划(基础研究-医学应用基础研究)项目(SKYD2023040). （基础研究-医学应用基础研究）
