军事医学2026，Vol.50Issue(6)：401-407,7.DOI:10.7644/j.issn.1674-9960.2026-00019
人胚胎干细胞诱导分化生成血管类器官的研究
Generation of blood vessel organoids from human embryonic stem cells
徐彩萍 1张博文 2李昀桥 2范韬 2陈彦州 2华筝 2覃金华 2李艳华1
作者信息
- 1. 安徽医科大学基础医学院,合肥 230032||军事科学院军事医学研究院,北京 100850
- 2. 军事科学院军事医学研究院,北京 100850
- 折叠
摘要
Abstract
Objective To establish and characterize vascular organoids based on the capacity of definitive differentiation and multiple cell self-organization of human embryonic stem cells(hESCs).Methods The hESCs were subjected to a stepwise induction protocol to sequentially differentiate into mesoderm progenitors,lateral plate mesoderm progenitors,and endothelial cells over a 5-day period.The resulting endothelial cell population was harvested and embedded in Matrigel to undergo three-dimensional culture of 6-8 days,allowing for self-organization into vascular organoids with branched luminal networks.The organoids were characterized via morphological analysis,flow cytometry,immunofluorescence staining,and transmission electron microscopy.Results The generated organoids formed complex networks of branched lumens,containing(41.10±4.51)%CD31+ endothelial cells and(6.88%±0.40)%CD140b+ pericytes.Among the endothelial cells,(43.00±0.21)%were CD31+CD184+ arterial endothelial cells and(3.66±0.25)%were CD31+CD184-CD73+ venous endothelial cells.Immunofluorescence analysis found that the vascular organoids were composed of VE-cadherin + Sox17 +arterial endothelial cells,VE-cadherin + NR2F2 + venous endothelial cells,PDGFR-β + pericytes,α-SMA + smooth muscle cells,and collagen I.Tight junctions were observed between endothelial cells,and characteristic Weibel-Palade body ultrastructures were identified within the cytoplasm that resembled the structures of human microvascular units.Conclusion We have established a robust method for generating hESCs-based vascular organoids that mimic the architecture of functional microvasculature.This three-dimensional vascular organoid can serve as a promising platform for research on vascular development and disease modeling.关键词
胚胎干细胞/人血管类器官/内皮细胞/周细胞Key words
embryonic stem cells/human blood vessel organoids/endothelial cells/pericytes分类
医药卫生引用本文复制引用
徐彩萍,张博文,李昀桥,范韬,陈彦州,华筝,覃金华,李艳华..人胚胎干细胞诱导分化生成血管类器官的研究[J].军事医学,2026,50(6):401-407,7.
