军事医学2026，Vol.50Issue(6)：425-433,9.DOI:10.7644/j.issn.1674-9960.2026-00010
青年男性作业疲劳血清脂质和免疫因子组学分析研究
Serum lipids and immune factor omics of operational fatigue in young males
张庆晗 1范利君 2宋明箫 2郭子逸 3王静 2陈学伟 2陆俊宇2
作者信息
- 1. 军事科学院军事医学研究院,天津 300050||北部战区疾病预防控制中心,沈阳 110034
- 2. 军事科学院军事医学研究院,天津 300050
- 3. 军事科学院军事医学研究院,天津 300050||武汉体育学院,武汉 430079
- 折叠
摘要
Abstract
Objective To study the molecular mechanisms underlying operational fatigue in young males using serum lipidomics and immune factor omics.Methods Sixty young males were involved in this study,who were asked to fill in the questionnaire about their fatigue and sleep during training period(TP)and recovery period(RP).The levels of blood lactic acid(BLA)and creatine kinase(CK)were measured to determine their levels of fatigue.Serum lipidomics and immune factor profiles were also analyzed to identify potential biomarkers that were detected using ELISA.Results Fatigue scores were significantly higher during TP than during RP(P<0.05)while sleep quality was poorer.CK[TP:(498.90±215.50)U/L vs.RP:(181.74±65.14)U/L]and BLA levels[TP:(5.83±1.23)mmol/L vs.RP:(3.07±0.64)mmol/L]were higher during TP.Lipidomics analysis suggested that the phosphatidylcholine(PC)subclass lipid molecule PC(18∶1-20∶3)+Na(AUC=0.85)was moderately correlated with fatigue scores(r=-0.64,P<0.001)and with BLA levels(r=0.57,P<0.001).Immune factor profiling indicated that platelet-derived growth factor BB(PDGF-BB)(AUC=0.78)was negatively correlated with BLA levels(r=-0.40,P<0.01)and CK(r=-0.46,P<0.001).The trends observed in the omics analyses were corroborated by ELISA validation results.Conclusion These findings suggest a"lipid-immune"two-dimensional evaluation system for operational fatigue.The lipid subclass PC and PDGF-BB may serve as candidate biomarkers for monitoring operational fatigue by reflecting changes in energy metabolism and responses related to minor tissue injury and repair.Furthermore,the PDGF-BB-PC metabolic axis may provide new insights into the mechanisms underlying the occurrence of operational fatigue.关键词
作业疲劳/脂质组学/免疫因子组学/生物标志物Key words
operational fatigue/lipidomics/immune factor omics/biomarkers分类
医药卫生引用本文复制引用
张庆晗,范利君,宋明箫,郭子逸,王静,陈学伟,陆俊宇..青年男性作业疲劳血清脂质和免疫因子组学分析研究[J].军事医学,2026,50(6):425-433,9.
