军事医学2026，Vol.50Issue(6)：434-440,7.DOI:10.7644/j.issn.1674-9960.2025-00255
黄河流域水体中病毒实时荧光定量PCR检测方法的建立及应用
A real-time quantitative PCR method for detection of viruses in water of the Yellow River basin
摘要
Abstract
Objective To establish a real-time quantitative PCR(qPCR)method for detection of common viruses in a water environment,and to assess the severity of contamination by norovirus,enterovirus,hepatitis A virus,and human adenovirus F in the Yellow River basin.Methods A qPCR method for detection of common viruses was established.Viruses were enriched via adsorption-elution,concentrated via ultrafiltration,and quantified by qPCR.Results Twenty-two samples were collected during the high-flow season(September-October 2024)and the low-flow season(March 2025).Neither norovirus nor enterovirus was detected in the samples.Hepatitis A virus was detected in 27.27%of the samples,and human adenovirus F in 18.18%.The concentrations of the detected viruses ranged from 1.3 to 2.1 lgGC/L.Conclusion Norovirus and enterovirus are not detected while contamination by hepatitis A virus and human adenovirus F is observed in some samples.These results may provide a reference for biosafety assessment and management of water resources in the Yellow River basin.关键词
黄河流域/水环境/病毒/实时荧光定量PCRKey words
the Yellow River basin/water environment/viruses/real-time quantitative PCR分类
医药卫生引用本文复制引用
杨璇璇,杨栋,庞心如,师丹阳,陈天姣,金敏,胡志勇,李君文..黄河流域水体中病毒实时荧光定量PCR检测方法的建立及应用[J].军事医学,2026,50(6):434-440,7.基金项目
"十四五"国家重点研发计划(2023YFC3205801) （2023YFC3205801）
