昆虫学报2026，Vol.69Issue(6)：804-814,11.DOI:10.16380/j.kcxb.2026.06.003
谷胱甘肽S-转移酶CsGSTσ1在桃小食心虫对高效氯氟氰菊酯解毒代谢中的作用研究
Role of the glutathione S-transferase CsGSTσ1 in the detoxification metabolism of Carposina sasakii(Lepidoptera:Carposinidae)to lambda-cyhalothrin
摘要
Abstract
[Aim]This study aims to explore the role of glutathione S-transferase(GST),which is upregulated in Carposina sasakii induced by lambda-cyhalothrin,in the detoxification metabolism of this insecticide,so as to lay the groundwork for further research on the role of GST in the metabolic resistance of C.sasakii.[Methods]Using the BLAST method,the coding sequences(CDSs)of GST genes in C.sasakii were identified based on the genome and transcriptome data.A phylogenetic tree of 14 GST proteins from C.sasakii and GST proteins from other insects was constructed using the neighbor-joining method.The median lethal concentration(LC50)value of lambda-cyhalothrin against the 3rd instar larvae of C.sasakii in 48 h was determined with drop method.At 48 h after exposure of the 3rd instar larvae of C.sasakii to LC50 of lambda-cyhalothrin,the GST activity in larvae was measured using a microplate reader with 1-chloro-2,4-dinitrobenzene(CDNB)and reduced glutathione(GSH)as substrates,and the expression levels of GST genes(CsGSTσ1,CsGSTσ2,CsGSTσ3,CsGSTσ4,CsGSTδ1,CsGSTδ4,CsGSTδ6,CsGSTω1,CsGSTω2,CsGSTω3,CsGSTω4,CsGSTε1,CsGSTζ2 and CsGSTζ3)were detected using RT-qPCR.The prokaryotic expression of CsGSTσ1,which was significantly induced and showed the highest expression level,was performed in vitro,and the enzyme kinetics of CsGSTσ1 was analyzed using a microplate reader.The metabolic activity of the recombinant CsGSTσ1 against lambda-cyhalothrin was analyzed by using ultra-high-performance liquid chromatography(UHPLC),and the antioxidant activity of CsGSTσ1 was measured using the disc diffusion assay.[Results]The phylogenetic tree showed that the 14 GSTs of C.sasakii belonged to the Delta,Sigma,Omega,Zeta and Epsilon families.The LC50 value of lambda-cyhalothrin against the 3rd instar larvae of C.sasakii in 48 h was 2.646 mg/L.At 48 h after induction with LC50 of lambda-cyhalothrin,the GST activity in the 3rd instar larvae of C.sasakii significantly increased and the expression level of CsGSTσ1 was significantly upregulated by 15.33-fold as compared to those of the control.The recombinant CsGSTσ1 had the Km value of(151.60±24.03)μmol/L and the Vmax value of(445.6±21.1)μmol/(min·mg),indicating good catalytic activity.The recombinant CsGSTσ1 exhibited metabolic activity against lambda-cyhalothrin.The disk diffusion assay results showed that CsGSTσ1 played a role in defending against oxidative stress damage.[Conclusion]CsGSTσ1 may be involved in the metabolism of lambda-cyhalothrin in C.sasakii.关键词
桃小食心虫/谷胱甘肽S-转移酶/高效氯氟氰菊酯/代谢抗性/原核表达/体外代谢Key words
Carposina sasakii/glutathione S-transferase/lambda-cyhalothrin/metabolic resistance/prokaryotic expression/in vitro metabolism分类
生物科学引用本文复制引用
左俊峰,方森森,丘彩云,黄彦娜,赵俊凝,彭雄,陈茂华..谷胱甘肽S-转移酶CsGSTσ1在桃小食心虫对高效氯氟氰菊酯解毒代谢中的作用研究[J].昆虫学报,2026,69(6):804-814,11.基金项目
陕西省重大科技专项(2020zdzx03-03-02) （2020zdzx03-03-02）
科技部对发展中国家援助项目(KY202002018) （KY202002018）
国家重点研发计划项目(2023YFD1600800) （2023YFD1600800）
联合国粮农组织/国际原子能机构CRP项目(26286) （26286）
