昆虫学报2026，Vol.69Issue(6)：862-871,10.DOI:10.16380/j.kcxb.2026.06.008
浙江湖州二化螟种群对七种杀虫剂的抗性监测及常用杀虫剂的田间药效评估
Resistance monitoring of Chilo suppressalis(Lepidoptera:Crambidae)populations to seven insecticides and field efficacy evaluation of common insecticides in Huzhou,Zhejiang,eastern China
摘要
Abstract
[Aim]This study aims to explore the resistance characteristics and mutation frequency of resistance sites of Chilo suppressalis populations in Huzhou City,Zhejiang Province,eastern China to common insecticides,and to systematically evaluate the control potential of new pesticides such as cyproflanilide through field trials,thereby providing a scientific basis for formulating resistance management strategies in single-cropping late rice regions of the Taihu Lake Basin.[Methods]The susceptibility of C.suppressalis populations to seven insecticides of four categories including diamides(chlorantraniliprole,cyproflanilide and broflanilide),diacylhydrazines(methoxyfenozide),milbemycins(spinetoram and abamectin),and organophosphates(triazophos)was determined using rice seedling dipping and capillary tube microapplication methods.The mutation sites in the ryanodine receptor gene of resistant individuals were analyzed through gene sequencing.A randomized plot trial was conducted to evaluate the field efficacy of seven insecticides,including 40％chlorantraniliprole·thiamethoxam water dispersible granule(WG),20％cyproflanilide suspension concentrate(SC),200 g/L tetraniliprole SC+5％abamectin emulsifiable concentrate(EC),10％abamectin·methoxyfenozide capsule suspension(CS),and 25％spinetoram WG,which were artificially sprayed during the booting stage of single-cropping late rice,against C.suppressalis.[Results]Resistance monitoring revealed that the Huzhou population of C.suppressalis exhibited high-level resistance to chlorantraniliprole(resistance ratio:278.0)and moderate resistance to spinetoram,methoxyfenozide,abamectin and triazophos,with the resistance ratios of 79.2,51.7,24.3 and 10.3,respectively.Multiple chlorantraniliprole resistance-associated mutations were identified in the ryanodine receptor gene.Testing of the ryanodine receptor gene revealed the mutation frequency of 21.1％at the Y4667C locus(all heterozygous)associated with chlorantraniliprole resistance,while the I4758M mutation frequency was as high as 84.2％.Field trials demonstrated that 20％cyproflanilide SC exhibited significantly superior plant protected efficacy(94.66％)and control efficacy against larvae(98.57％)to other agents(plant protected efficacy and control efficacy against larvae:80.85％and 85.93％for 25％spinetoram WG,66.43％and 71.87％for 200 g/L tetraniliprole SC+5％abamectin EC,and 63.84％and 58.83％for 10％abamectin·methoxyfenozide CS,respectively)when applied once at the booting stage of single-cropping late rice.Application of 40％chlorantraniliprole·thiamethoxam WG during the rice flowering stage reduced residual C.suppressalis populations by 58.3％relative to its application at the rice booting stage.[Conclusion]Huzhou C.suppressalis populations has developed severe resistance to chlorantraniliprole.Immediate discontinuation of this insecticide in northern Zhejiang rice areas is recommended,with rotational use strategies for moderately resistant agents.Cyproflanilide shows potential as a superior alternative,and a dual-application strategy("booting stage+post-flowering stage")is proposed to enhance control efficacy sustainability against the 3rd-generation C.suppressalis in the Taihu Lake Basin.关键词
二化螟/抗性监测/抗性治理/环丙氟虫胺/田间防治效果Key words
Chilo suppressalis/resistance monitoring/resistance management/cyproflanilide/field efficacy分类
生物科学引用本文复制引用
吕进,王思洁,郭芳睿,乔松涛,史锦华,倪爽,何琴玲,高聪芬,吴顺凡..浙江湖州二化螟种群对七种杀虫剂的抗性监测及常用杀虫剂的田间药效评估[J].昆虫学报,2026,69(6):862-871,10.基金项目
湖州市科技项目(2023GZ40) （2023GZ40）
浙江省农业农村厅团队项目(浙农科发(2023)13号) （浙农科发(2023）
国家自然科学基金面上项目(32272576) （32272576）
