临床传统医学和药理学（英文）2026，Vol.7Issue(2)：60-69,10.DOI:10.1016/j.ctmp.2026.200279
尼日利亚传统天然碱与钾碱对子宫平滑肌的作用及Wistar大鼠体内组织病理学与免疫组织化学效应
Traditional Nigerian natron and potash:Effects on uterine smooth muscle and their histopathology and immunohistochemical responses in Wistar rats
Vincent Agu 1Okezie Emmanuel 2Sonachi Nnedimma Okeke 3Peter Achukwu 1Solomon Nnah Ijioma4
作者信息
- 1. Department of Medical Laboratory Sciences,University of Nigeria,Nsukka 400006,Enugu State,Nigeria
- 2. Department of Animal Sciences,College of Food,Agricultural and Environmental Sciences,The Ohio State University,Wooster,OH 44691,USA||Department of Biochemistry,Abia State University,Uturu PMB 2000,Abia State,Nigeria
- 3. Department of Biochemistry,Abia State University,Uturu PMB 2000,Abia State,Nigeria
- 4. Department of Zoology and Environmental Biology,Michael Okpara University of Agriculture,Umudike 440109,Umuahia,Abia State,Nigeria
- 折叠
摘要
Abstract
Background:Natron(sodium carbonate)and potash(potassium carbonate)are traditionally used by birth atten-dants in Nigeria to facilitate labor and manage menstrual disorders.However,their uterine activity and potential risks to uterine integrity remain poorly characterized.
Objective:This study evaluated the effects of natron and potash on uterine smooth muscle contractility and examined associated histopathological and immunohistochemical changes in rat uterine tissue.
Methods:The cationic composition(Na+,K+,Ca2+)of different natron varieties and potash was quantified.Uterine contractility was assessed in vitro using isolated rat uterine smooth muscle preparations in organ baths.Substances with contractile activity underwent acute toxicity testing before in vivo evaluation.Fifty adult female Wistar rats(nulliparous and multiparous)were randomized into ten groups and administered 5％or 10％natron or potash for eight weeks.Uterine tissues were analysed histologically and immunohistochemically using progesterone receptor and Vimentin markers.
Results:Potash and one natron variety shared similar electrolyte profiles and elicited significant uterine contrac-tions,whereas other natron varieties induced smooth muscle relaxation.Chronic exposure resulted in marked uterine remodelling,including altered endometrial-to-myometrial ratios,glandular hyperplasia,fibrocollagenous stromal deposition,and inflammatory infiltration.Progesterone receptor and Vimentin expression increased in a concentration-dependent manner,with greater immunoreactivity observed in nulliparous rats.
Conclusion:Natron exhibits heterogeneous uterine effects that are strongly influenced by its cationic composition.While some varieties and potash demonstrate uterotonic activity consistent with traditional use,the associated structural remodelling and inflammatory changes highlight potential adverse uterine consequences,underpinning the need for rigorous safety evaluation prior to clinical application.关键词
钾碱/天然碱/宫缩促进剂/宫缩抑制剂/子宫/平滑肌Key words
Potash/Natron/Uterotonic/Tocolytic/Uterine/Smooth muscle引用本文复制引用
Vincent Agu,Okezie Emmanuel,Sonachi Nnedimma Okeke,Peter Achukwu,Solomon Nnah Ijioma..尼日利亚传统天然碱与钾碱对子宫平滑肌的作用及Wistar大鼠体内组织病理学与免疫组织化学效应[J].临床传统医学和药理学（英文）,2026,7(2):60-69,10.
