临床传统医学和药理学（英文）2026，Vol.7Issue(2)：101-111,11.DOI:10.1016/j.ctmp.2026.200290
Majoon falasfa及其改良制剂在Wistar大鼠中的神经药理学评价及基于高效液相色谱的植物化学相关性研究
Neuropharmacological evaluation and HPLC-based phytochemical correlation of Majoon falasfa and its modified formulations in Wistar rats
摘要
Abstract
Background:Age-related cognitive disorders like amnesia and Alzheimer's disease(AD)impair productivity and quality of life,prompting exploration of phytomedicine as a safer alternative to nootropic agents. Objective:This study compared the neuropharmacological effects of Unani classical sugar-based Majoon-e-Falasafa(MF)with redesigned sugar-free tablets:powder-based tablets(TP),aqueous extract-based tablets(TAE),and hy-droalcoholic extract-based tablets(THAE)in rats,and aimed to relate these effects to their high performance liq-uid chromatography(HPLC)characteristics. Methods:Three sugar-free tablet formulations were prepared by omitting sugar from the traditional pharma-copoeial formula of MF and incorporating approved additives,followed by compression to produce TP,TAE,and THAE.Each 20 μL sample of the four tablet formulations was subjected to HPLC analysis on a Shimadzu LC-20 AD system with a detection wavelength of 254 nm.Wistar rats were randomly assigned to treatment groups receiving oral doses of MF(1166.67 mg/kg),TP(303.33 mg/kg),TAE(291.35 mg/kg),THAE(303.27 mg/kg),or 0.3％carboxymethyl cellulose as vehicle control,administered twice daily for 7 consecutive days.Neuropharmacolog-ical parameters assessed included gross behavioural observation,phenobarbitone-induced narcosis potentiation,spontaneous motor activity,neuromuscular coordination,central analgesic effect,anticonvulsant activity,condi-tioned avoidance response,and three validated maze models evaluating learning acquisition,working memory,and spatial working memory. Results:HPLC analysis revealed distinct compositional differences:TP was dominated by early-eluting polar constituents,TAE showed enrichment of mid-retention peaks,while THAE exhibited a broad distribution of peaks spanning early,mid,and late retention times,indicating the presence of diverse phytoconstituents.All formulations exhibited a favorable safety profile(LD50＞2000 mg/kg),without mortality or abnormal be-havioural changes observed during the 14-day acute toxicity test.THAE consistently exhibited the most pro-nounced pharmacological effects across all paradigms tested:it significantly increased spontaneous locomotor activity(P＜0.01),prolonged phenobarbitone-induced sleep duration(P＜0.01),reduced the extensor phase of maximal electroshock-induced convulsions(P＜0.01),and exerted the most potent central analgesic effect with reaction times of(9.67±0.67)s at 60 min and(9.83±1.37)s at 80 min(P＜0.01).THAE also showed the greatest suppression of the conditioned avoidance response(66.6％)and superior cognitive-enhancing effects,significantly attenuating scopolamine-induced memory impairment:it reduced transfer latency in the elevated plus maze(P＜0.001),achieved a 79.8％success rate in the radial arm maze,and increased the spontaneous alternation percentage to 74.14％in the Y-maze(P＜0.01). Conclusion:The findings suggest that THAE possesses superior central nervous system(CNS)activity,likely attributable to its enriched and diverse phytochemical profile.The observed effects may involve modulation of central neurotransmission and neuroprotective mechanisms;however,these remain hypothesis-generating.This study highlights the importance of extraction methodology in optimizing the therapeutic potential of polyherbal formulations and supports further mechanistic and clinical investigations.关键词
多组分草药制剂/Majoon-e-Falasafa/高效液相色谱/片剂/中枢神经调节/学习记忆/尤纳尼医学Key words
Polyherbal formulation/Majoon-e-Falasafa/HPLC/Tablet/CNS modulation/Learning behaviour and memory(maze models)/Unani medicine引用本文复制引用
Tajuddin,Aziz ur Rahman,Qazi Zaid Ahmad,Mohammad Rashid,Syed Shariq Mian..Majoon falasfa及其改良制剂在Wistar大鼠中的神经药理学评价及基于高效液相色谱的植物化学相关性研究[J].临床传统医学和药理学（英文）,2026,7(2):101-111,11.基金项目
The authors state that the study was funded by grants from the CCRUM,Ministry of AYUSH(Grant Number F.No.3-27/2014-CCRUM/Tech.,Date-07-04.2015)(2015-2018),New Delhi,which is duly acknowledged. （Grant Number F.No.3-27/2014-CCRUM/Tech.,Date-07-04.2015）
