生物技术通报2026，Vol.42Issue(6)：1-12,12.DOI:10.13560/j.cnki.biotech.bull.1985.2025-0743
甘薯DNA甲基转移酶和去甲基化酶基因家族的全基因组鉴定与表达分析
Genome-wide Identification and Expression Analysis of DNA Methyltransferase and Demethylase Gene Family in Sweetpotato
摘要
Abstract
[Objective]Identification and analysis of the gene family members,its structures and expression patterns of DNA methyltransferase(IbC5-MTase)and demethylase(IbdMTase)will provide theoretical support for in-depth exploration of the molecular basis and biological functions of DNA methylation in sweetpotato.[Method]The physicochemical properties,sequences,evolution and structures of the IbC5-MTase and IbdMTase gene family members were analyzed by bioinformatic methods.The expressions of IbC5-MTase and IbdMTase in different sweetpotato tissues,and under abiotic and biotic stress conditions were analyzed by integrating transcriptome data with RT-qPCR.[Result]A total of seven IbC5-MTase genes and four IbdMTase genes were identified in the whole genome of sweetpotato,distributed across 8 linkage groups.The IbC5-MTase and IbdMTase proteins contain 355-1 305 amino acids(aa),with theoretical isoelectric points(pI)ranging from 4.81 to 9.81.Subcellular localization predictions indicate that nine proteins are in the nucleus.Phylogenetic analysis showed the IbC5-MTase and IbdMTase proteins are classified into four and three subfamilies,respectively.Conserved motif analysis demonstrated distinct motif compositions between two protein types.Promoter regions of both gene families were enriched with cis-regulatory elements associated with light responsiveness,hormonal regulation,and stress adaptation.Tissue-specific expression profiling revealed relatively high expression levels of IbDRM2 and IbCMT3b,with overall higher expression of both gene families in aerial tissues as compared to roots,particularly in young tissues.Transcriptome analysis under abiotic stresses demonstrated widespread expression reprogramming of these genes during salt,drought,and cold adaptation.Stem nematode infection triggered distinct transcriptional responses,IbCMT3a and IbDRM2 were significantly downregulated,whereas IbCMT3b and IbROS1 were upregulated,as validated by RT-qPCR.[Conclusion]The IbC5-MTase and IbdMTase genes of sweetpotato have potential functions under biotic and abiotic stresses.关键词
甘薯/C5-MTase及dMTase基因/生物信息学/生物及非生物胁迫/表达模式Key words
sweetpotato/C5-MTase and dMTase genes/bioinformatics/biotic and abiotic stresses/expression profile引用本文复制引用
张艾岑,闫会,马居奎,张允刚,李强..甘薯DNA甲基转移酶和去甲基化酶基因家族的全基因组鉴定与表达分析[J].生物技术通报,2026,42(6):1-12,12.基金项目
徐州农科院基金(JC2024001),国家自然科学基金项目(32301842),国家甘薯产业技术体系(CARS-10) （JC2024001）
