生物技术通报2026，Vol.42Issue(6)：13-21,9.DOI:10.13560/j.cnki.biotech.bull.1985.2025-0856
木薯MeMYB106基因克隆及其在叶片花青素合成中的功能
Cloning of MeMYB106 in Cassava and Its Function in Anthocyanin Biosynthesis of Leaves
摘要
Abstract
[Objective]MYB transcription factors are primarily participate in the biosynthesis of flavonoids and anthocyanins.To explore MYB transcription factors in regulating anthocyanin biosynthesis in cassava leaves and verify its biological function would provide a theoretical basis for elucidating the molecular mechanisms of anthocyanin synthesis and leaves color improvement in cassava(Manihot esculenta Crantz).[Method]The coding sequence of MeMYB106 was cloned using cDNA from South China 9(SC9)leaves as a template.Transient expression in tobacco was performed to determine its subcellular localization.RT-qPCR was applied to analyze the expression patterns of MeMYB106 in different tissues and differently colored leaves.Virus-induced gene silencing(VIGS)was employed to investigate the gene function in anthocyanin synthesis.Yeast one-hybrid assays and dual luciferase reporter assay were used to examine the regulatory relationship between MeMYB106 and anthocyanidin reductase(MeANR)gene.[Result]A MeMYB106 gene was cloned from SC9 leaves,with a coding region of 1 194 bp encoding 398 amino acids.The subcellular localization revealed that MeMYB106 was localized in the nucleus.Tissue-specific expression analysis showed that MeMYB106 was highly expressed in SC9 leaves,axillary buds,and stems,with significantly higher expression in green-leaves varieties compared with purple-leaves varieties.Silencing MeMYB106 in SC9 resulted in purple pigmentation in newly emerged leaves,with varying degrees of purple pigmentation depending on silencing efficiency.The anthocyanin content in the leaves of silenced plants was significantly higher than that in empty vector control.Further analysis demonstrated that MeMYB106 bound to the promoter region of MeANR to enhance its expression,thereby influencing anthocyanin biosynthesis in cassava leaves.[Conclusion]MeMYB106 transcription factor in cassava negatively regulate the biosynthesis of anthocyanins,and MeMYB106 positively regulate the expression of MeANR.Silencing MeMYB106 promotes anthocyanin accumulation in cassava leaves.关键词
木薯/MeMYB106转录因子/花青素/基因克隆/功能分析Key words
cassava/MeMYB106 transcription factor/anthocyanin/gene cloning/functional analysis引用本文复制引用
安飞飞,罗秀芹,蔡杰,薛晶晶,朱文丽..木薯MeMYB106基因克隆及其在叶片花青素合成中的功能[J].生物技术通报,2026,42(6):13-21,9.基金项目
国家重点研发计划(2023YFD1200200),热带作物生物育种全国重点实验室科研项目(NKLTCBCXTD30,NKLTCBCXTD31),中央级公益性科研院所基本科研业务费专项资金(1630052023016,1630032024002) （2023YFD1200200）
