生物技术通报2026，Vol.42Issue(6)：22-30,9.DOI:10.13560/j.cnki.biotech.bull.1985.2025-0701
马铃薯GARP转录因子家族分析及StARR14-X1基因功能验证
Analysis of the Potato GARP Transcription Factor Family and Functional Validation of StARR14-X1
摘要
Abstract
[Objective]To identify key genes regulating anthocyanin accumulation in the tubers of colored potatoes(Solanum tuberosum L.),provide genetic resources and technical references for potato molecular breeding.[Method]GARP transcription factors were screened based on transcriptome sequencing data of purple potato tubers at different developmental stages.Bioinformatics analysis was conducted,and functional verification of StARR14-X1 gene related to anthocyanin regulation was performed.[Result]The 12 GARP transcription factors were screened through transcriptome data.The amino acid sequence lengths of 12 GARP transcription factors were concentrated between 148-686 aa,the molecular weight ranged from 16.66 to 75.44 kD,the pI value ranged from 5.13 to 8.05,the fatty index ranged from 71.36 to 97.36,and the instability coefficient ranged from 35.59 to 75.94,all of which belonged to hydrophilic proteins.Subcellular localization prediction showed that 12 GARP transcription factors were distributed in the nucleus,cytoplasm and chloroplasts.The StARR14-X1 gene was successfully transformed into tetraploid tobacco using the Agrobacterium-mediated leaf disc method,and transgenic tobacco plants overexpressing StARR14-X1 were obtained.The anthocyanin contents in the stems and petals of StARR14-X1-overexpressing transgenic lines increased significantly.[Conclusion]StARR14-X1 gene has a positive regulatory function in anthocyanin accumulation and promotes anthocyanin accumulation.关键词
马铃薯/花青素/GARP转录因子/正调控Key words
potato/anthocyanin/GARP transcription factor/positive regulation引用本文复制引用
武小娟,赵雪雯,王沛捷,聂虎帅,李楠,马宇,杨恩泽,马杰茹,马艳红..马铃薯GARP转录因子家族分析及StARR14-X1基因功能验证[J].生物技术通报,2026,42(6):22-30,9.基金项目
内蒙古农业大学基本科研业务费专项(BR22-11-16),内蒙古科学技术研究院产业技术创新项目(2024RCYJ04004),内蒙古自治区马铃薯产业技术创新推广体系(IMARS-08-P03),内蒙古自治区自然科学基金(2024QN03070) （BR22-11-16）
