生物技术通报2026，Vol.42Issue(6)：40-52,13.DOI:10.13560/j.cnki.biotech.bull.1985.2025-1472
马铃薯LEA_2基因家族鉴定及响应低温与激素功能分析
Identification and Functional Analysis of the LEA_2 Gene Family in Potato in Response to Cold Stress and Hormones
摘要
Abstract
[Objective]Late embryogenesis abundant proteins(LEA)are important genes that regulate plant growth and development as well as responses to abiotic stress.This study aimed to investigate the biological function of StLEA_2 genes,so as to provide a theoretical basis and genetic resources for improving potato tolerance to cold.[Method]Members of the LEA_2 gene family were identified from the potato genome,and their physicochemical properties,subcellular localization,gene structure,evolutionary features and cis-acting elements were analyzed.Transcriptome and RT-qPCR analyses were employed to examine the expression patterns of StLEA_2 under cold stress,jasmonic acid(JA)and gibberellic acid(GA)treatments.The function of StLEA37 in cold stress response was analyzed through Agrobacterium-mediated transient overexpression in tobacco.[Result]A total of 45 StLEA_2 genes were identified in cultivated potato(Solanum tuberosum L.),while 42 ScLEA_2 genes were identified in the cold-tolerant wild species(Solanum commersonii),all of them were classified into nine subgroups.Subcellular localization prediction indicates that members of the LEA_2 gene family are mainly localized in the cytoplasm and chloroplasts.Collinearity analysis revealed that there are 10 and 11 pairs of homologous genes in the LEA family of cultivated and wild potatoes,respectively.The promoters of StLEA_2 genes contain numerous light-,stress-,and hormone-responsive elements.Expression analysis indicated that 44 StLEA_2 genes are involved in responses to low-temperature stress.Under GA and JA treatments,the expressions of 39 and 43 genes,respectively,changed significantly.StLEA37 is localized on the plasma membrane,and its transient overexpression contributes to the regulation of plant cold stress tolerance by activating the COR-CBF regulatory pathway and reactive oxygen species scavenging mechanisms.[Conclusion]A total of 45 StLEA_2 genes were identified in the potato genome.StLEA37 is localized on the plasma membrane and may participate in plant responses to cold stress by affecting the COR-CBF pathway and ROS scavenging mechanisms,as well as by mediating GA and JA signaling pathways.关键词
马铃薯/LEA_2基因家族/低温胁迫/茉莉酸/赤霉素/表达分析Key words
potato/LEA_2 gene family/cold stress/jasmonic acid/gibberellic acid/expression analysis引用本文复制引用
段雅馨,李成晨,潘玉玲,王丽,安康,李小波,祝光涛,刘计涛..马铃薯LEA_2基因家族鉴定及响应低温与激素功能分析[J].生物技术通报,2026,42(6):40-52,13.基金项目
国家重点研发计划项目(YS2023YFD1200005-5),广东省基础与应用基础研究重大项目(2021B0301030004),广东省基础与应用基础研究基金项目(2023A1515012611) （YS2023YFD1200005-5）
