生物技术通报2026，Vol.42Issue(6)：64-76,13.DOI:10.13560/j.cnki.biotech.bull.1985.2025-1350
马铃薯核心MADS-box转录因子进化特性与功能预测
Evolutionary Characteristics and Functional Inferences of Core MADS-box Transcription Factors in Potato
摘要
Abstract
[Objective]The MADS-box transcription factor family is widely involved in plant growth,development,and floral organ formation.This study systematically analyzes the function of core MADS-box transcription factors across 51 potato genomes,aiming to provide a theoretical basis and genetic resources for molecular breeding in potato(Solanum tuberosum L.).[Method]Based on whole-genome data from 33 wild and 18 cultivated diploid potatoes,core MADS-box genes were identified.Bioinformatics analysis was conducted to investigate their physicochemical properties,phylogenetic relationships,chromosomal distribution,and conserved domains.Expression patterns were analyzed by integrating transcriptome data and RT-qPCR.Their biological functions were inferred through GO enrichment analysis,exogenous hormone treatment,and comparison with functionally characterized homologous genes from major crops.Collinearity analysis was also performed on closely related species.[Result]A total of 95 common MADS-box genes,referred to as core genes,were identified across 51 diploid potatoes,classified into two major subfamilies:Type I(Mα,Mβ,Mγ)and Type II(MIKC*,MIKCc).Physicochemical analysis indicated that these proteins are generally hydrophilic and unstable.Phylogenetic and structural analyses revealed that each subfamily possesses characteristic motifs and domains.Chromosomal mapping showed that the genes are clustered on chromosomes Chr01,Chr04,Chr05,and Chr12.Expression pattern analysis indicated that several MIKCc-type genes are specifically expressed in floral buds,stolons,and other tissues.GO enrichment analysis demonstrated that this family is involved in floral organ development and hormone response processes.Hormone response analysis revealed that the expressions of key candidate genes are suppressed by GA₃ and induced by ABA.Phylogenetic comparison with functional genes such as OsMADS29 from rice suggested that some genes may participate in tuber development and yield formation.Collinearity analysis revealed 37 conserved MADS-box genes between potato and its close relatives,with some genes exhibiting translocation variations.[Conclusion]The MADS-box gene family in potatoes retains conserved functions in regulating floral and tuber development during evolution.Candidate genes such as DM8C02G24490.1 and DM8C03G29590.1,which are associated with seed development and tuber formation,provide important targets for subsequent functional validation and breeding improvement.关键词
马铃薯/转录因子/MADS-box/基因家族/块茎发育Key words
potato/transcription factor/MADS-box/gene family/tuber development引用本文复制引用
董亚瑞,栗锦烨,崔庆晗,贾玉鑫..马铃薯核心MADS-box转录因子进化特性与功能预测[J].生物技术通报,2026,42(6):64-76,13.基金项目
国家自然科学基金青年科学基金项目(32402608),云南省基础研究计划面上项目(202501AT070005) （32402608）
