生物技术通报2026，Vol.42Issue(6)：98-106,9.DOI:10.13560/j.cnki.biotech.bull.1985.2025-1343
木薯采后生理变质相关基因MeGLYI-13上游转录因子的筛选
Screening of Upstream Transcription Factors of MeGLYI-13 Gene Related to Postharvest Physiological Deterioration of Cassava
摘要
Abstract
[Objective]Cassava(Manihot esculenta Crantz)is one of the most important economic and food crops in the world.The problem of postharvest physiological deterioration(PPD)causes a serious impact on the storage period and economic benefits.As a member of the glyoxalaseⅠ(GLYⅠ)family,the expressionof MeGLYI-13 was positively correlated with the PPD tolerance of cassava.In-depth analysis of the molecular mechanism of upstream regulation of MeGLYI-13 involved in cassava PPD tolerance may provide a new idea for exploring the molecular mechanism of cassava postharvest physiological deterioration process,and PPD-tolerant cassava germplasm innovation.[Method]The promoter region sequence of MeGLYI-13 was cloned and analyzed.The proMeGLYI-13-pAbAi bait vector was constructed and transformed into yeast cells to obtain the bait strain.The expression pattern analysis and interaction verification of candidate transcription factors were carried out by yeast one-hybrid screening library experiment using cassava cDNA library.[Result]The 2 000 bp upstream promoter sequence of MeGLYI-13 was successfully cloned.The sequence contained cis-acting elements responding to low temperature,drought,auxin,gibberellin,light,defense and stress.Through yeast one-hybrid screening,four candidate transcription factors were screened from cassava cDNA library,which were ethylene-responsive transcription factor ERF1 and AT-hook motif nuclear localization proteins AHL17,AHL29 and AHL31.The rotary verification test and dual luciferase test further confirmed that MeAHL17 played a negative regulatory role in the promoter activity of MeGLYI-13.Gene expression analysis showed that the expression trend of MeAHL17 was negatively correlated with PPD tolerance of cassava.[Conclusion]The combination of MeAHL17 and MeGLYI-13 promoter regulate gene expression,which may play a role in the physiological deterioration of cassava after harvest.关键词
木薯/采后生理变质/乙二醛酶/转录因子/启动子/AT-hook基序核定位蛋白Key words
cassava/postharvest physiological deterioration/glyoxalase/transcription factors/promoter/AT-hook motif nuclear localization protein引用本文复制引用
沈晨,车延年,丁中平,王祥文,葛玉建,符东青,周亚星乔,李瑞梅..木薯采后生理变质相关基因MeGLYI-13上游转录因子的筛选[J].生物技术通报,2026,42(6):98-106,9.基金项目
海南省自然科学基金项目(323RC537),三亚崖州湾科技城"崖州湾"菁英人才项目(SCKJ-JYRC-2024-81),三亚市科技专项(2024KJFX014),中国热带农业科学院国家热带农业科学中心科技创新团队(CATASCXTD202301),中央公益性科研院所基本科研业务费(1630032025010),热带作物生物育种全国重点实验室科研项目(NKLTCBCXTD39) （323RC537）
