中国生物防治学报2026，Vol.42Issue(3)：528-545,18.DOI:10.16409/j.cnki.2095-039x.2025.11.016
环境因素驱动纹彩猎蝽雌虫肠道菌群结构与多样性显著差异
Structure and Diversity of Gut Microbiota in Female Euagoras plagiatus Depend Largely on Environmental Factors
摘要
Abstract
The gut bacterial community plays a crucial role throughout the developmental stages of host insects.In this study,based on the PacBio platform and using full-length 16S rDNA amplicon sequencing technology,we systematically analyzed the gut bacterial community characteristics of female Euagoras plagiatus under different living environments.The results revealed that 650 and 239 operational taxonomic units(OTUs)were detected in the guts of wild-type(Y)and laboratory-reared(Z)female E.plagiatus,respectively,with 218 OTUs shared between the two groups.Both Alpha and Beta diversity analyses indicated that the gut bacterial community of wild-type individuals exhibited higher richness and diversity.Furthermore,through bacterial isolation techniques,19 bacterial strains belonging to 6 different species were successfully isolated from the gut of female E.plagiatus.Quantitative real-time PCR(RT-qPCR)analysis further revealed significant differences in the abundance of endogenous bacteria between wild-type and reared insects.This study elucidates the influence of environmental factors on the composition and diversity of the gut bacterial community in female E.plagiatus adults,providing an important theoretical basis for a deeper understanding of their environmental adaptation mechanisms.关键词
纹彩猎蝽/肠道细菌群落/生存环境/菌群多样性/高通量16S rDNA测序Key words
Euagoras plagiatus/gut bacterial community/living environment/bacterial diversity/high-throughput 16S rDNA sequencing分类
农业科技引用本文复制引用
李昊,丘明艳,张颖昕,黄孙滨,刘浩宇,肖艳松,刘婷,金丰良,许小霞..环境因素驱动纹彩猎蝽雌虫肠道菌群结构与多样性显著差异[J].中国生物防治学报,2026,42(3):528-545,18.基金项目
中国烟草总公司湖南省公司科技项目(HN2024KJ06,CZYC2023JS06) （HN2024KJ06,CZYC2023JS06）
