中国生物防治学报2026，Vol.42Issue(3)：600-610,11.DOI:10.16409/j.cnki.2095-039x.2026.02.003
基因组精简和启动子工程相结合的策略提升福莱菌肽产量
Combining Genome Streamlining and Promoter Engineering Strategies to Construct High-Yield Strain for Fabclavines
摘要
Abstract
Fabclavines produced by Xenorhabdus budapestensis strain XBD8 exhibit broad-spectrum antimicrobial activity,showing significant potential for application in the control of plant disease.However,their low yield and high application costs have hindered industrial development.This study aimed to construct high-yield Fabclavine-producing strains through a combined strategy of genome reduction and promoter engineering.Genome mining of Xenorhabdus budapestensis strain XBD8 using the bioinformatics tool antiSMASH identified 11 secondary metabolite biosynthetic gene clusters(BGCs)in addition to the Fabclavine BGC.Iterative knockout of core biosynthetic genes from these BGCs generated a streamlined chassis strain,designated FRT12.Notably,gene2204,involved in the biosynthesis of Fabclavines Fcl-7 and Fcl-8,was identified during this genome reduction process.Promoter engineering strategy was applied to replace the promoters of genes fclC and gene2204,leading to the successful construction of the dual-promoter replacement strain MPW9.The yield of Fabclavines was increased from 73.4 mg/L to 121.3 mg/L.By swapping these two promoters in the streamlined chassis strain FRT12,the constructed strain MPW11 demonstrated further improved Fabclavines production,reaching 142.6 mg/L in shake-flask fermentation.These findings establish a foundation for industrial-scale production of Fabclavines.关键词
福莱菌肽/布达佩斯致病杆菌/基因组精简/启动子工程Key words
fabclavines/Xenorhabdus budapestensis/genome streamlining/promoter engineering分类
农业科技引用本文复制引用
王玉乐,尹长琰,贾丰莲,任杰,李广悦..基因组精简和启动子工程相结合的策略提升福莱菌肽产量[J].中国生物防治学报,2026,42(3):600-610,11.基金项目
国家重点研发计划(2023YFA0914700) （2023YFA0914700）
新疆维吾尔自治区重点研发计划项目(2022B02044) （2022B02044）
中央级公益性科研院所基本科研业务费专项(Y2025YC19) （Y2025YC19）
区域协同创新发展专项(25CXQJ002) （25CXQJ002）
