中国生物防治学报2026，Vol.42Issue(3)：611-620,10.DOI:10.16409/j.cnki.2095-039x.2026.02.014
蒙氏肠球菌ZC-05对猕猴桃细菌性溃疡病防效评价及生防机制初探
Evaluation of Control Efficacy of Enterococcus mundtii ZC-05 against Bacterial Canker of Kiwifruit and Preliminary Study on Its Biocontrol Mechanism
摘要
Abstract
Kiwifruit bacterial canker,often termed the"cancer"of kiwifruit,currently lacks effective control methods.In this study,a potential biocontrol strain Enterococcus mundtii ZC-05,previously isolated from stem of Kiwifruit,was labeled with green fluorescent protein(GFP)to investigate its colonization characteristics.Results indicated that protoplast fusion was an effective method for transforming strain ZC-05,successfully yielding the GFP-labeled transformant ZC-05-GFP.Confocal microscopy observation revealed that ZC-05-GFP colonized kiwifruit stems two weeks after trunk injection and reach the leaf via the sap flow.Field trials demonstrated that injection of ZC-05 suspension significantly reduced the incidence of kiwifruit bacterial canker,achieving a control efficacy of 50.96％,which was substantially higher than that of the copper-based bactericide Thiodiazole-Copper(13.46％)(P＜0.05).Whole-genome sequencing of ZC-05 identified seven genes encoding type Ⅵ secretion system(T6SS)effector proteins.Furthermore,quantitative real-time PCR analysis indicated that the gene Gm-1574,encoding an antibacterial toxic protein,was significantly up-regulated after co-culture with Pseudomonas syringae pv.actinidiae(Psa-Y),the pathogen of Kiwifruit bacterial canker,suggesting its potential important role in the biocontrol activity of ZC-05.This study clarifies the colonization characteristics of ZC-05,confirms its field efficacy against kiwifruit bacterial canker,and preliminarily elucidates its biocontrol mechanisms.These findings provide a basis for the development and practical application of trunk-injected biocontrol agents in the future.关键词
猕猴桃细菌性溃疡病/蒙氏肠球菌/荧光标记/生防机制Key words
kiwifruit bacterial canker/Enterococcus mundtii/GFP labeling/biocontrol mechanism分类
农业科技引用本文复制引用
顾佳颖,尹志诚,李南羿,蔡小剑,张昕..蒙氏肠球菌ZC-05对猕猴桃细菌性溃疡病防效评价及生防机制初探[J].中国生物防治学报,2026,42(3):611-620,10.基金项目
国家自然科学基金(31970093) （31970093）
