癌变·畸变·突变2026，Vol.38Issue(4)：261-269,9.DOI:10.3969/j.issn.1004-616x.2026.04.001
E2F5调控自噬在α粒子辐射诱发人支气管上皮细胞恶性转化中的作用
E2F5 mediated autophagy in α-particle radiation-induced malignant transformation of human bronchial epithelial cells
摘要
Abstract
OBJECTIVE:To investigate the role of E2F transcription factor 5(E2F5)in regulating autophagy during α-particle radiation-induced malignant transformation of human bronchial epithelial cells.METHODS:Immortalized human bronchial epithelial BEP2D cells,early transformed RH24 cells(the 24 th generation after α-particle irradiation),and malignant transformed BERP35T-1 cells were used.Western blot was performed to detect E2F5 protein expression in the three cell lines.BERP35T-1 cells with the highest malignancy were selected and divided into five groups:blank control,negative control,rapamycin(Rapa),E2F5 silencing,and E2F5 silencing combined with Rapa.The E2F5 silencing and combined groups were transfected with E2F5 siRNA at a final concentration of 10 nmol/L for 24 h;the negative control group was transfected with an equal concentration of negative control sequence;and the Rapa groups were treated with 25 pmol/L autophagy activator rapamycin for 24 h.Western blot was used to detect the autophagy marker protein LC3B expression.Cell viability,wound healing rate,and the number of invasive cells were detected by CCK-8,wound healing,and Transwell invasion assays,respectively.RESULTS:Compared with BEP2D cells,E2F5 protein expression was significantly upregulated in both RH24 and BERP35T-1 cells(P<0.01).After E2F5 silencing in BERP35T-1 cells,the LC3B-Ⅱ/Ⅰ ratio decreased from 1.00±0.04 to 0.51±0.04,cell viability dropped from(97.01±1.99)%to(34.96±1.26)%,the wound healing rate at 24 h decreased from(19.18±0.17)%to(5.61±2.16)%,at 48 h from(54.17±2.72)%to(13.14±3.20)%,and the number of invaded cells decreased from 225.67±8.14 to 42.67±0.58(all P<0.01).Rapamycin intervention reversed these effects,as evidenced by an increase in LC3B-Ⅱ/Ⅰ ratio from 0.69±0.02 to 1.43±0.05,cell viability from(69.44±4.45)%to(100.73±4.28)%,wound healing rate at 24 h from(7.36±1.51)%to(13.82±1.02)%,at 48 h from(22.84±0.13)%to(39.11±0.61)%,and the number of invaded cells from 90.67±7.77 to 241.67±44.86(P<0.05 or P<0.01).CONCLUSION:During α-particle radiation-induced malignant transformation of BEP2D cells,E2F5 protein expression was significantly upregulated.Increased E2F5 activated autophagy,thereby enhancing cell viability,migration,and invasion,and consequently promoting the process of α-particle radiation-induced malignant transformation.关键词
E2F转录因子5/自噬/α粒子/人支气管上皮细胞/恶性转化/肺癌Key words
E2F5/autophagy/alpha particle/human bronchial epithelial cell/malignant transformation/lung cancer分类
医药卫生引用本文复制引用
杨佳文,邵帅,李辰,王成芳,周密,曲功霖,苟巧..E2F5调控自噬在α粒子辐射诱发人支气管上皮细胞恶性转化中的作用[J].癌变·畸变·突变,2026,38(4):261-269,9.基金项目
国家自然科学基金(81000862) （81000862）
