北方园艺Issue(14)：8-17,10.DOI:10.11937/bfyy.20254456
矮牵牛游离小孢子培养体系的建立及关键影响因素分析
Establishment of an Isolated Microspore Culture System and Analysis of Key Influencing Factors in Petunia hybrida
摘要
Abstract
Taking four Petunia hybrida genotypes,namely'Mitchell''Rose Star Spot New Superb''Mirage Exquisite Pink'and'Dreamy Burgundy'as experimental materials,the method of isolated microspore culture was employed to investigate the impacts of factors including the correspondence between the external morphology of flower buds and the development stages of microspores,disinfection methods,genotype differences,low-temperature pretreatment,combined pretreatment of starvation and high-temperature,and hormone treatment on the effects of isolated microspore culture.The objective was to establish an isolated microspore culture system for Petunia hybrida,in order to provide a reference for the haploid breeding research of Petunia hybrida and other Solanaceae ornamental plants.The results showed that the sampling periods for the four varieties corresponded to petal-sepal ratios of 0.48,0.73,0.60,and 0.58 respectively.There were significant differences in induction effects among different genotypes,with'Mitchell'having the highest induction rate.Disinfection with 1％NaClO for 25 minutes could reduce the contamination rate of microspores and maintain their high activity.Low-temperature pretreatment(treatment at 4 ℃ for 6 days)and combined pretreatment of starvation and high-temperature(3 days high-temperature treatment at 32 ℃+6 days starvation treatment)could significantly increase the induction rate of embryoids.Hormone combinations of TDZ at 0.5 mg·L-1 and 2,4-D at 0.5 mg·L-1+6-BA at 0.2 mg·L-1 could promote the formation and development of embryoids.关键词
矮牵牛/游离小孢子/胚状体/胁迫预处理/激素调控Key words
Petunia hybrida/isolated microspore/embryoid/stress pretreatment/hormone regulation分类
农业科技引用本文复制引用
王姣姣,张彦锋,袁黎,刘曼,田树娟..矮牵牛游离小孢子培养体系的建立及关键影响因素分析[J].北方园艺,2026,(14):8-17,10.基金项目
中央引导地方科技发展资助项目(ZYYD2024ZY02). （ZYYD2024ZY02）
