北方园艺Issue(14)：85-93,9.DOI:10.11937/bfyy.20254399
豇豆轮纹病拮抗细菌筛选及其生防效果评价
Screening of Antagonistic Bacteria Against Cowpea Ring Spot and Evaluation of Biocontrol Efficacy
摘要
Abstract
Taking the tissues of cowpea plants and the rhizosphere soil as the test materials,the microorganisms were isolated and purified by the dilution plating method.Combined with the plate confrontation culture method,the antagonistic strains were initially screened with the target of the cowpea target spot pathogen.The re-screening was completed by the determination of the broad-spectrum of inhibition,and the control effect of the antagonistic strains on cowpea target spot was evaluated by the detached leaf method,in order to provide a reference for the biological control of cowpea target spot.The results showed that antibacterial rate exceeding 70％against cowpea ring spot.WZ-9 showed an antibacterial rate of 76.57％.After broad-spectrum antifungal activity of WZ-9 revealed that exhibited strong inhibitory activity against all ten tested plant pathogenic fungi.Based on morphological and molecular identification,the strain was identified as Pseudomonas aeruginosa.Further assays indicated that its cell-free fermentation filtrate achieved a 50％inhibition rate against the pathogen.In detached leaf assays,WZ-9 provided a control efficacy of 44.5％against cowpea ring spot.In conclusion,after screening successfully identified P.aeruginosa strain WZ-9,which demonstrates dual functionality by antagonizing various cowpea pathogens and promoting seedling growth.These findings position WZ-9 as a promising candidate for developing new biopesticides.关键词
豇豆轮纹病/多主棒孢霉/铜绿假单胞菌/生物防治Key words
cowpea ring spot/Corynespora cassiicola/Pseudomonas aeruginosa/biological control分类
农业科技引用本文复制引用
沙文榕,张晓琳,曾琳琳,潘磊,牛蒙亮,乾义柯..豇豆轮纹病拮抗细菌筛选及其生防效果评价[J].北方园艺,2026,(14):85-93,9.基金项目
湖北省教育厅科学技术研究计划重点资助项目(D20234405) （D20234405）
江汉大学校级科研资助项目(2023KJZX39). （2023KJZX39）
