大豆科学2026，Vol.45Issue(4)：1-10,10.DOI:10.11861/j.issn.1000-9841.2026.04.0001
菜用大豆生育期功能基因KASP标记的开发与验证
Development and Validation of KASP Markers for Functional Genes Controlling Maturity in Vegetable Soybean
摘要
Abstract
To establish an efficient marker-assisted selection system in vegetable soybean,this study targeted variant loci within key genes regulating maturity and developed seven corresponding Kompetitive Allele-Specific PCR(KASP)markers,covering the J,E1-E4,GmPRR3a and GmPRR3b.A total of 290 vegetable soybean accessions representing diverse maturity groups were phenotyped and genotyped at two ecologically contrasting sites:Changping,Beijing(long-day temperate condition)and Sanya,Hainan(short-day tropical condition).Associations between marker genotypes and maturity-related traits,including days from emergence to initial flowering(VE-R1),days to full seed stage(VE-R6),and days to initial maturity(VE-R7)were systematically analyzed.Field evaluation revealed that in Beijing,the average days to flowering,full seed stage and initial maturity were 43.5±12.5 d,103.3±20.9 d and 118.3±19.4 d,respectively;in Hainan,the corresponding values were 29.1±2.4 d,61.3±4.0 d and 81.2±6.7 d,respectively.Genotype-phenotype association analysis confirmed that all seven KASP markers reliably distinguished homozygous and heterozygous genotypes.Predictive accuracy for maturity traits varied across developmental stages and environments:accuracy for the first flowering stage ranged from52.1%to63.4%,while higher accuracy(64.1%to 81.7%)was observed for the full-seed stage,the critical phase determining commercial pod quality and yield.Notably,four markers,E1(G/C),E2(A/T),GmPRR3a(T/A)and GmPRR3b(C/T),demonstrated particularly strong predictive performance for the full seed stage under Hainan's conditions.Haplotype analysis further clarified the phenotypic effects of major allelic variants:specific alleles,including E1-C,E2-T,GmPRR3a-A and GmPRR3b-T,were significantly associated with early flowering and early maturity.The functional KASP marker system established in this study enables early and precise assessment of maturity traits in vegetable soybean,providing a reliable technical platform for molecular marker-assisted selection and designed breeding.关键词
菜用大豆/生育期/功能标记/KASP/MASKey words
vegetable soybean/maturity/functional marker/Kompetitive Allele-Specific PCR(KASP)/Marker-Assisted Selection(MAS)引用本文复制引用
杨帆,王培国,孙艳辉,张繁繁,孙石,韩天富,杜吉到,袁珊..菜用大豆生育期功能基因KASP标记的开发与验证[J].大豆科学,2026,45(4):1-10,10.基金项目
山东省重点研发计划(农业良种工程)(2026LZGC024) （农业良种工程）
国家重点研发计划(2023YFD1201300) （2023YFD1201300）
三亚中国农业科学院国家南繁研究院"南繁专项"(YBXM2428). （YBXM2428）
