福建农业学报2026，Vol.41Issue(5)：535-541,7.DOI:10.19303/j.issn.1008-0384.2026.05.002
青海省288份小麦品种(系)多酚氧化酶活性基因分布检测
Distribution of PPO-related Genes in 288 Wheat Cultivars from Qinghai Province
摘要
Abstract
[Objective]Distribution of the genes related to brown discoloration-causing polyphenol oxidase(PPO)in wheat cultivars of Qinghai Province was investigated by molecular marker analysis.[Method]Genomic DNA was extracted from the leaves of 288 wheat cultivars from Qinghai at the two-leaf stage.Functional markers PPO18,PPO16,and PPO29 were applied for genotyping the Ppo-A1 and Ppo-D1 loci,with the 685 bp and 876 bp amplicons corresponding to the Ppo-A1a and Ppo-A1b alleles,respectively,and the 713 bp and 490 bp amplicons corresponding to the Ppo-D1a and Ppo-D1b alleles,respectively.PCR products were separated by 1.5%agarose gel electrophoresis,and the distribution frequencies of each allele and allelic combinations were statistically analyzed.[Result]In the cultivars,the allelic frequency of Ppo-A1a was 25.3%and that of Ppo-A1b 20.8%,while those of Ppo-D1a and Ppo-D1b 58.0%and 19.8%,respectively.The appearing frequencies of the combinations Ppo-A1b/Ppo-D1a,Ppo-A1a/Ppo-D1b,Ppo-A1a/Ppo-D1a,and Ppo-A1b/Ppo-D1b were 13.2%,6.9%,14.6%,and 8.3%,respectively.[Conclusion]The Ppo-A1 locus was largely characterized by the high-PPO-activity Ppo-A1a allele with the frequency of 25.3%,whereas Ppo-D1 was mainly represented by the low-activity Ppo-D1a allele of 58.0%.Of the biallelic combinations,the medium-activity genotype Ppo-A1a/Ppo-D1a of 14.6%and the low-activity genotype Ppo-A1b/Ppo-D1a of 13.2%dominated the genetic profile.The molecular information on the Qinghai wheat cultivars could facilitate the breeding of varieties with reduced PPO activity to enhance the quality and extend shelf life of the flour made from them.关键词
小麦/多酚氧化酶/分子检测/遗传多样性/青海省Key words
wheat/polyphenol oxidase/molecular detection/genetic diversity/Qinghai Province分类
农业科技引用本文复制引用
霍营营,王昕,侯万伟,张小娟..青海省288份小麦品种(系)多酚氧化酶活性基因分布检测[J].福建农业学报,2026,41(5):535-541,7.基金项目
青海省"昆仑英才·高端创新创业人才"项目(2022-2025年) （2022-2025年）
