福建农业学报2026，Vol.41Issue(5)：542-549,8.DOI:10.19303/j.issn.1008-0384.2026.05.003
OsHSP70-2及其互作蛋白OsPR10a调控水稻稻瘟病功能解析
Preliminary Study on Blast Resistance Regulated by OsHSP70-2 and Interacting OsPR10a in Rice
摘要
Abstract
[Objective]Roles of heat-shock HSP70 and its interactive protein in regulating blast resistance of rice plants were analyzed.Previously identified OsHSP70-2 and its interacting OsPR10a from rice plants with durable resistance to blast disease were used as a reference.[Methods]An OsHSP70-2 mutant was constructed by phylogenetic analysis and gene editing to measure the lesion area and fungal biomass for a phenotypic analysis.Roles and relationship between OsHSP70-2 and OsPR10a was studied using bimolecular fluorescence complementation and luciferase complementary imaging experiments.Cell death induced by the proteins was verified on a tobacco system and by a double luciferase transient expression assay.[Results]There were certain species-specific variations in the motif of OsHSP70-2,but a high degree of conservation existed in the overall protein structure.In vitro,the OsHSP70-2 mutant rice plants obtained by gene editing were more sensitive to Magnaporthe oryzae inoculation.An interaction between OsHSP70-2 and OsPR10a was confirmed,and their co-expression,not OsPR10a alone,caused tobacco cell death.[Conclusion]OsHSP70-2 might regulate the nuclease activity expression through appropriate folding,stability,or subcellular localization of PR10a to activate the downstream defense response.The preliminarily revealed molecular mechanism by this study indicated that OsHSP70-2 mediated the homeostasis of rice plants through regulating OsPR10a.The information would substantially facilitate the breeding of disease resistant varieties.关键词
水稻/热休克蛋白/稻瘟病/HSP70/水稻病程相关蛋白10aKey words
rice/heat shock protein/blast/HSP70/PR10a分类
农业科技引用本文复制引用
陈婷,吴方喜,谢华安,张建福,李昆阳,魏毅东,朱永生,陈丽萍,何炜,郑燕梅,蒋家焕,蔡秋华..OsHSP70-2及其互作蛋白OsPR10a调控水稻稻瘟病功能解析[J].福建农业学报,2026,41(5):542-549,8.基金项目
国家水稻产业技术体系建设专项(CARS-01-08) （CARS-01-08）
福建省科技重大专项(2024NZ029027) （2024NZ029027）
福建省农业科学院优秀科技创新人才专项(YCZX202401) （YCZX202401）
