福建农业学报2026，Vol.41Issue(5)：582-587,6.DOI:10.19303/j.issn.1008-0384.2026.05.008
玉米茎基腐病菌LAMP检测方法的建立
LAMP Assay for Rapid Fusarium verticillioides Detection on Corn Plant
摘要
Abstract
[Objective]A rapid,sensitive,and visual loop-mediated isothermal amplification(LAMP)-based method to detect Fusarium verticillioides of corn stalk rot was developed.[Method]A set of primers based on the Elongation factor-1α sequence of F.verticillioides,together with the template DNA from infected corn leaves and optimized temperature and time,were applied for the LAMP assay.Specificity and sensitivity of the prototype method were tested to verify the assay applicability for accurate pathogen detection on field specimens.[Result]Allowing a reaction time of 60 min at 65℃,the assay effectively and specifically detected F.verticillioides on the diseased corn plants.It had a detection limit of 10 fg·μL-1 and provided results consistent with the morphological diagnosis.On 35 field-collected corn plants that were suspected of stalk rot infection,the LAMP assay,as well as the physiological identification,found 17 to be F.verticillioides-positive and 18 negative.[Conclusion]The newly established LAMP assay could visually,rapidly,specifically,and accurately detect F.verticillioides on the infected corn plants.It was adequate for monitoring and early diagnosis to facilitate effective control of the disease.关键词
玉米/茎基腐病/拟轮枝镰孢菌/环介导等温核酸扩增技术(LAMP)/检测Key words
corn/corn stalk rot/Fusarium verticillioides/loop-mediated isothermal amplification/detection分类
农业科技引用本文复制引用
侯翔宇,黄鹏,张杰,曾兆华,余德亿,李本金,姚锦爱..玉米茎基腐病菌LAMP检测方法的建立[J].福建农业学报,2026,41(5):582-587,6.基金项目
福建省科技计划公益类专项(2023R1022004、2023R1071、2022R10240010) （2023R1022004、2023R1071、2022R10240010）
福建省农业高质量发展超越"5511"协同创新工程项目(XTCXGC2021011、XTCXGC2021017) （XTCXGC2021011、XTCXGC2021017）
