福建农业学报2026，Vol.41Issue(5)：596-604,9.DOI:10.19303/j.issn.1008-0384.2026.05.010
新疆4个地区传统酸马奶中微生物菌株的分离及遗传进化分析
Isolation and Phylogenetic Analysis of Microbes Isolated from Xinjiang Koumiss
摘要
Abstract
[Objective]Microorganisms in the traditional koumiss sampled from 4 regions in Xinjiang were isolated and identified.This was of importance for enriching local microbial data.[Method]30 samples of koumiss were collected from Aheqi,Gejiagou,Wusu and Nalati.Microbes including lactic acid bacteria and yeasts in samples were isolated and purified through plate cultivation method with morphological characteristics observed.Then the amplification and sequencing of their rDNA/ITS gene fragments and phylogenetic analysis were carried out.[Result]Seventy strains,which included 64 bacteria and 6 yeasts belonging to 18 species,7 families,and 12 genera,were isolated.Of them,10 strains from Aheqi belonged to one family,2 genera,and 4 species,including Bacillus cereus,Bacillus albus,Heyndrickxia oleronia and Heyndrickxia ginsengihumi;25 strains from Gejiagou belonged to 6 families,10 genera,and 9 species,including Lentilactobacillus kefiri,Lactobacillus helveticus,Lactiplantibacillus plantarum,Companilactobacillus crustorum,Acetobacter pasteurianus,Leuconostoc mesenteroides,Enterococcus durans,Monosporozyma unispora and Pichia ethanolica;7 strains from Wusu belonged to one family,one genus,and 4 species,including Bacillus cereus,Bacillus albus,Bacillus paramycoides and Bacillus wiedmannii;and 28 strains from Nalati belonged to 6 families,7 genera,and 9 species,including Lentilactobacillus kefiri,Lacticaseibacillus paracasei,Lactobacillus helveticus,Lactiplantibacillus plantarum,Acetobacter pasteurianus,Acetobacter malorum,Monosporozyma unispora,Pichia ethanolica and Pichia fermentans.Among all the strains,Enterococcus durans constituted the dominant species,accounting for 17.14%,while Lacticaseibacillus paracasei and Lactobacillus helveticus comprised 14.29%,respectively,and the others collectively made up 54.28%.[Conclusion]The 70 strains isolated from 30 samples of koumiss were identified with taxological classifications.Among them,there were a total of 39 strains of lactic acid bacteria including Lentilactobacillus kefiri,Lacticaseibacillus paracasei,Lactobacillus helveticus,Lactiplantibacillus plantarum,Companilactobacillus crustorum,Leuconostoc mesenteroides and Enterococcus durans,6 strains of yeasts,and 25 strains of other microorganisms.The information would aid further studies on probiotics and other health-related functions of the traditional product.关键词
新疆/酸马奶/微生物/分离/鉴定Key words
Xinjiang/koumiss/microorganisms/isolation/identification分类
生物科学引用本文复制引用
王俊伟,佘笑兰,柳梦婕,高天晴,努尔·库尔玛那里,阮永磊,郭会玲,刘志强..新疆4个地区传统酸马奶中微生物菌株的分离及遗传进化分析[J].福建农业学报,2026,41(5):596-604,9.基金项目
新疆维吾尔自治区现代农业产业技术体系项目(XARS-09-202610) （XARS-09-202610）
新疆维吾尔自治区重点研发计划项目(2022B02027-1) （2022B02027-1）
