广东农业科学2026，Vol.53Issue(5)：92-107,16.DOI:10.16768/j.issn.1004-874X.2026.05.008
月季、玫瑰FLA基因家族鉴定及其在雌雄蕊中的表达分析
Identification of the FLA Gene Family in Rosa chinensis and R.rugosa and Analysis of Its Expression in Pistils and Stamens
摘要
Abstract
[Objective]Fasciclin-like arabinogalactan proteins(FLAs)were a subclass of arabinogalactan-proteins(AGPs).The FLAs were widely involved in biological processes such as plant growth and development,sexual reproduction,and abiotic stress response.Identifying and analyzing the FLA gene family in Rosa plants(R.chinensis and R.rugosa)could help to understand the role of the FLA gene family in the biological processes of Rosa plants.[Method]This study employed bioinformatics to conduct a genome-wide identification of the FLA gene family in R.chinensis and R.rugosa.The FLA gene families of the two species were compared,and the expression patterns of FLA genes in the stamens and pistils of R.chinensis and R.rugosa were analyzed.[Result]Twenty-five FLA genes were identified in both R.chinensis and R.rugosa,unevenly distributed across their respective seven chromosomes.Analysis of the structure of FLA genes in R.chinensis and R.rugosa revealed that 18 RcFLA in R.chinensis and 21 RrFLA in R.rugosa did not contain introns.Analysis of cis-regulatory elements found that light response elements,MeJA response elements,and auxin-related cis-elements were widely distributed.Among all 50 FLA proteins,33 had signal peptides at their N-termini,30 were transmembrane protein,and all 44 were likely localized on the cell membrane.The closely related FLA proteins showed strong similarity in their two-dimensional and three-dimensional structures and motif distribution.Phylogenetic analysis divided all FLAs into six groups,with Group Ⅰ having the most members and branches,and other groups containing FLAs from both R.chinensis and R.rugosa.Analysis of the expression patterns of FLA genes in the stamens and pistils of R.chinensis and R.rugosa revealed that some FLA were specifically highly expressed in the stamens or pistils.[Conclusion]A total of 25 FLA genes were identified in both R.chinensis and R.rugosa,indicating that these FLA genes are conserved and also species-specific.Additionally,some FLA genes were specifically highly expressed in the pistil or stamen,and were potentially involved in the fertility regulation of R.chinensis and R.rugosa,providing a reference for further research on the regulation of reproductive development in Rosa plants.关键词
月季/玫瑰/类成束阿拉伯半乳糖蛋白/基因家族/育性Key words
Rosa chinensis/Rosa rugosa/fasciclin-like arabinogalactan proteins/gene family/fertility分类
农业科技引用本文复制引用
侯秋梅,张佳佳,杨朔,周艳,朱立,吴洪娥..月季、玫瑰FLA基因家族鉴定及其在雌雄蕊中的表达分析[J].广东农业科学,2026,53(5):92-107,16.基金项目
贵州省科技计划项目(黔科合平台KXJZ[2025]036) （黔科合平台KXJZ[2025]036）
贵州省植物园青年基金(黔植科合[2023]04号) （黔植科合[2023]04号）
贵州特色经济植物育种创新能力建设项目(黔科合服企[2024]004-1) （黔科合服企[2024]004-1）
