广东医学2026，Vol.47Issue(7)：1024-1029,6.DOI:10.13820/j.cnki.gdyx.20260037
骨钙素在血管钙化中的作用机制
Mechanisms of osteocalcin in vascular calcification
摘要
Abstract
Objective To investigate the role and underlying mechanisms of osteocalcin in vascular calcification.Methods Forty healthy female Sprague-Dawley rats were randomly assigned to five groups(n=8 per group):normal group,sham-operated plus calcification group,ovariectomy plus calcification group,osteocalcin-silencing group(ovar-iectomy+calcification+osteocalcin silencing),and negative control silencing group(ovariectomy+calcification+negative control).Except for the normal group,vascular calcification was induced in all groups by vitamin D3 administra-tion.Osteocalcin gene silencing was performed in the osteocalcin-silencing group.Serum lipid profiles were measured.Vascular calcification was evaluated by Von Kossa staining.The mRNA and protein expression levels of osteocalcin(bone Gla protein,BGP)and runt-related transcription factor 2(Runx2)were determined using reverse transcription quantita-tive polymerase chain reaction(RT-qPCR)and Western blot analysis,respectively.Results Compared with the nor-mal group,low-density lipoprotein cholesterol(LDL-C)levels were significantly increased in all calcification groups,including the sham-operated plus calcification,ovariectomy plus calcification,osteocalcin-silencing,and negative con-trol groups(P＜0.05).Von Kossa staining demonstrated marked medial vascular calcification in all calcification groups compared with the normal group.Osteocalcin silencing significantly attenuated vascular calcification compared with the negative control group(P＜0.05).The mRNA and protein expression levels of BGP and Runx2 were significantly in-creased in all calcification groups compared with the normal group(P＜0.05).Following osteocalcin silencing,both BGP and Runx2 mRNA and protein expression levels were significantly reduced compared with the negative control group(P＜0.05).Conclusion Osteocalcin participates in the development and progression of vascular calcification and may pro-mote vascular calcification by upregulating Runx2 expression.关键词
骨钙素/血管钙化/骨转录因子-2Key words
osteocalcin/vascular calcification/runt-related transcription factor 2分类
医药卫生引用本文复制引用
苗立坤,杨伟,陈素芬,李胜军,金焱,刘宏,陈章荣..骨钙素在血管钙化中的作用机制[J].广东医学,2026,47(7):1024-1029,6.基金项目
国家自然科学基金资助项目(81960085) （81960085）
大理市科技局基金项目(2019KGB017) （2019KGB017）
