国际生殖健康/计划生育杂志2026，Vol.45Issue(4)：290-293,325,5.DOI:10.12280/gjszjk.20260051
激光辅助孵化改善隐匿精子症患者低等级卵裂胚囊胚形成并妊娠一例
Laser-Assisted Hatching Improves Blastocyst Formation from Low-Grade Cleavage Embryos in Patients with Cryptozoospermia and Achieves Pregnancy:A Case Report
唐雪琼 1吴晓丹 1李登坤 1张静 1苏果1
作者信息
- 1. 236000 安徽医科大学附属阜阳人民医院生殖医学科
- 折叠
摘要
Abstract
In assisted reproductive technology,the low-grade cleavage embryo(LGCE)is associated with a lower probability of developing into viable blastocysts,making it difficult to achieve satisfactory outcomes.We report a case of a 35-year-old male patient with cryptozoospermia who sought medical attention after more than two years of unprotected intercourse without conception while attempting to have a second child.He was diagnosed with secondary male infertility.His 31-year-old spouse underwent assisted reproductive technology with him.In the first oocyte retrieval cycle,16 oocytes were retrieved,and 10 underwent intracytoplasmic sperm injection(ICSI).On Day 3,all embryos were LGCE(Grade Ⅲ-Ⅳ),and no viable blastocysts were formed following blastocyst culture.In the second oocyte retrieval cycle,18 oocytes were retrieved,and 11 underwent ICSI.On Day 3,the embryos remained LGCE.Two fresh embryos were transferred,but did not result in pregnancy.The remaining embryos underwent blastocyst culture,and laser-assisted hatching(LAH)was employed to improve LGCE blastocyst formation,ultimately resulting in one 3BC-grade blastocyst that was cryopreserved.A live birth was subsequently achieved following a frozen-thawed embryo transfer cycle.By summarizing the management of this patient's LGCE,we explore the laboratory strategy to improve the blastocyst formation of LGCE in patients with cryptozoospermia,and to enhance their chances of pregnancy.关键词
激光辅助孵化/低等级卵裂胚/隐匿精子症/精子注射,细胞质内/妊娠Key words
Laser-assisted hatching/Low-grade cleavage embryo/Cryptozoospermia/Sperm injections,intracytoplasmic/Pregnancy引用本文复制引用
唐雪琼,吴晓丹,李登坤,张静,苏果..激光辅助孵化改善隐匿精子症患者低等级卵裂胚囊胚形成并妊娠一例[J].国际生殖健康/计划生育杂志,2026,45(4):290-293,325,5.
