河北工业科技2026，Vol.43Issue(4)：289-299,11.DOI:10.7535/hbgykj.2026yx04001
基于标准汤剂的马兰草配方颗粒质量评价研究
Study on quality evaluation of Kalimeris indica(L.)Sch.-Bip.formula granules based on standard decoction
摘要
Abstract
To achieve quality control throughout the entire production process of Kalimeris indica(L.)Sch.-Bip.formula granules,a quality evaluation method based on standard decoction was established for Kalimeris indica(L.)Sch.-Bip.formula granules.According to the preparation requirements of standard decoction for traditional Chinese medicine,15 batches of Kalimeris indica(L.)Sch.-Bip.standard decoction and three batches of formula granules were prepared.The contents of cryptochlorogenic acid,caffeic acid,ferulic acid,3,5-O-di-caffeoylquinic acid,and 4,5-O-di-caffeoylquinic acid were quantitatively calculated,and the characteristic chromatogram of Kalimeris indica(L.)Sch.-Bip.was established and analyzed using ultra-high performance liquid chromatography.The results show that there were 23 common peaks in the UPLC fingerprint spectra of 15 batches of Kalimeris indica(L.)Sch.-Bip.standard decoction(S1-S15)and three batches of formula granules(KL1-KL3).Similarity evaluation,HCA,and PCA indicate that the components of Kalimeris indica(L.)Sch.-Bip.formula granules are similar to those of the standard decoction.The extract yield of 15 batches of Kalimeris indica(L.)Sch.-Bip.standard decoction(S1-S15)range from 18.84%to 32.52%.The mass fractions of cryptochlorogenic acid,caffeic acid,ferulic acid,3,5-O-di-caffeoylquinic acid,and 4,5-O-di-caffeoylquinic acid are 0.42～0.83,0.37～0.95,0.29～0.61,0.08～0.18,and 0.19～0.44 mg/g,respectively.The transfer rates of the five components from the decoction pieces to the standard decoction are 33.77%～82.62%,28.44%～65.96%,30.98%～71.08%,26.29%～77.87%,and 25.91%～60.79%,respectively.The transfer rates of the five components from the decoction pieces to the formula granules in the three batches of Kalimeris indica(L.)Sch.-Bip.formula granules(KL1-KL3)are 47.96%～50.82%,32.89%～39.45%,42.48%～43.89%,26.94%～33.96%,and 33.32%～35.57%,respectively,which are basically consistent with the transfer rates from the decoction pieces to the standard decoction.The proposed preparation process of Kalimeris indica(L.)Sch.-Bip.standard decoction is feasible,and the established characteristic fingerprint spectrum and content determination methods of the standard decoction are stable,providing a basis for the quality standard research of Kalimeris indica(L.)Sch.-Bip.formula granules.关键词
中药化学/马兰草/标准汤剂/配方颗粒/指纹图谱/层次聚类分析/主成分分析/质量评价Key words
chemistry of traditional Chinese medicine/Kalimeris indica(L.)Sch.-Bip./standard decoction/formula granules/fingerprint spectrum/hierarchical clustering analysis(HCA)/principal component analysis/quality evaluation分类
医药卫生引用本文复制引用
何燕玲,向家俊,安加文,梁玉婷,刘珈羽,陈浩,李慧馨..基于标准汤剂的马兰草配方颗粒质量评价研究[J].河北工业科技,2026,43(4):289-299,11.基金项目
贵州省科合计划项目(黔科合支撑[2022]一般186) （黔科合支撑[2022]一般186）
