河北医学2026，Vol.32Issue(7)：1077-1083,7.DOI:10.3969/j.issn.1006-6233.2026.07.04
羌活醇对类风湿性关节炎大鼠OPG/RANKL/RANK通路及骨稳态的影响
Effect of Notopterol on the OPG/RANKL/RANK Pathway and Bone Homeostasis in Rats with Rheumatoid Arthritis
摘要
Abstract
Objective:To investigate the effect of notopterol on the osteoprotegerin(OPG)/receptor ac-tivator of nuclear factor-κB ligand(RANKL)/receptor activator of nuclear factor-κB(RANK)pathway and bone homeostasis in rats with rheumatoid arthritis(RA).Methods:Rats were randomly divided into 6 groups(12 rats per group):control group,RA group,low-dose notopterol group,medium-dose notopterol group,high-dose notopterol group,and tripterygium glycosides group.RA model was constructed in all groups but the control group.After successful modeling and administration,rats in the low,medium,and high-dose no-topterol groups received intraperitoneal injections of 3.33,6.66,and 13.32mg/kg of notopterol,respective-ly;rats in the Tripterygium glycosides group received 9mg/kg of Tripterygium glycosides by gavage;and rats in the control group and RA group received an equal volume of normal saline via the same routes.All treat-ments were administered once daily for 4 consecutive weeks.The following indicators were detected:Changes in toe swelling,arthritis index,mechanical pain threshold,and thermal withdrawal latency;serum levels of tumor necrosis factor-α(TNF-α),interleukin-1β(IL-1β),interleukin-17(IL-17),tartrate-resistant acid phosphatase 5b(TRAP-5b),C-terminal telopeptide of type I collagen(CTX-I),bone-specific alka-line phosphatase(BALP),and N-terminal propeptide of type I procollagen(PINP)by ELISA;pathological changes of ankle joint tissue by HE staining;number of osteoclasts in ankle joint tissue by tartrate-resistant acid phosphatase 5b(TRAP-5b)staining;mRNA and protein expressions of OPG,RANKL,RANK,and nuclear factor-κB p65 subunit(NF-κB p65)in ankle joint tissue by qRT-PCR and Western blot,respec-tively.Results:Compared with the control group,the RA group showed severe inflammatory lesions of arthri-tis,with significant increases in toe swelling,arthritis index,serum levels of TNF-α,IL-1β,IL-17,TRAP-5b,and CTX-I,number of osteoclasts in ankle joint tissue,mRNA and protein expressions of RANKL and RANK,mRNA expression of NF-κB p65,and the protein ratio of phosphorylated NF-κB p65(p-NF-κB p65)to total NF-κB p65(P<0.05).In contrast,the mechanical pain threshold,thermal withdrawal laten-cy,serum levels of BALP and PINP,and mRNA and protein expressions of OPG in ankle joint tissue were significantly decreased in the RA group(P<0.05).Compared with the RA group,the low-dose,medium-dose,and high-dose notopterol groups,as well as the tripterygium glycosides group,had the opposite trends in the above indicators(P<0.05).Conclusions:Notopterol may restore bone homeostasis in RA rats by reg-ulating the OPG/RANKL/RANK pathway.关键词
类风湿性关节炎/羌活醇/骨稳态/炎症/骨吸收/骨生成Key words
Rheumatoid arthritis/Notopterol/Bone homeostasis/Inflammation/Bone resorp-tion/Bone formation引用本文复制引用
李青松,宋丽园,谢大伟..羌活醇对类风湿性关节炎大鼠OPG/RANKL/RANK通路及骨稳态的影响[J].河北医学,2026,32(7):1077-1083,7.基金项目
河北省医学科学研究课题计划资助(20261658) （20261658）
