河北医学2026，Vol.32Issue(7)：1084-1090,7.DOI:10.3969/j.issn.1006-6233.2026.07.05
miR-655-3p调节DDX3X对IL-1β诱导的骨关节炎软骨细胞损伤的影响
Effect of miR-655-3p on IL-1β-Induced Chondrocyte Injury in Osteoarthritis by Regulating DDX3X
摘要
Abstract
Objective:To investigate the role of microRNA-655-3p(miR-655-3p)in interleukin-1β(IL-1β)-induced chondrocyte injury in osteoarthritis,and to analyze its association with DEAD-box RNA helicase 3X-linked(DDX3X).Methods:A chondrocyte injury model was established by inducing human chondrocyte line CHON-001 with IL-1β.Chondrocytes were randomly assigned into control group,model group,model+miR-NC group,model+miR-655-3p mimics group,model+sh-NC group,model+sh-DDX3X group,model+miR-655-3p mimics+pcDNA3.1 group,and model+miR-655-3p mimics+pcDNA3.1-DDX3X group.qRT-PCR was used to detect the expression of miR-655-3p and DDX3X mRNA;MTT assay was used to detect CHON-001 cell proliferation;TUNEL staining was used to detect cell apoptosis;ELISA was used to detect the levels of IL-6,TNF-α,and IL-10;Western blot was used to detect the protein ex-pression of Bcl-2,Bax,and DDX3X;the relationship between miR-655-3p and DDX3X was validated using dual luciferase reporter assay.Results:Compared with the control group,the model group showed decreases in miR-655-3p,IL-10,and Bcl-2,and raises in DDX3X,cell proliferation inhibition rate,apoptosis rate,IL-6,TNF-α,and Bax(P<0.05).Compared with the model group and model+miR-NC group,the model+miR-655-3p mimics group showed raises in miR-655-3p,IL-10,and Bcl-2,and reductions in DDX3X,cell proliferation inhibition rate,apoptosis rate,IL-6,TNF-α,and Bax(P<0.05).Compared with the model group and model+sh-NC group,the model+sh-DDX3X group showed no significant difference in miR-655-3p(P>0.05),but reductions in DDX3X,cell proliferation inhibition rate,apoptosis rate,IL-6,TNF-α,and Bax,and increases in IL-10,and Bcl-2(P<0.05).Compared with the model+miR-655-3p mim-ics group and model+miR-655-3p mimics+pcDNA3.1 group,the model+miR-655-3p mimics+pcDNA3.1-DDX3X group showed no significant difference in miR-655-3p(P>0.05),but increases in DDX3X,cell proliferation inhibition rate,apoptosis rate,IL-6,TNF-α,and Bax,and reductions in IL-10 and Bcl-2(P<0.05).There was a targeting relationship between miR-655-3p and DDX3X.Conclusions:Overexpres-sion of miR-655-3p can inhibit DDX3X,thereby improving IL-1β-induced chondrocyte injury in osteoar-thritis.关键词
骨关节炎/miR-655-3p/DDX3X/IL-1β/软骨细胞Key words
Osteoarthritis/miR-655-3p/DDX3X/IL-1β/Chondrocyte引用本文复制引用
刘立冰,祝丰成,李海欣,李焕龙,蔡怡..miR-655-3p调节DDX3X对IL-1β诱导的骨关节炎软骨细胞损伤的影响[J].河北医学,2026,32(7):1084-1090,7.基金项目
昆明市卫生健康委员会卫生科研课题项目(2025-04-07-005) （2025-04-07-005）
