河北医学2026，Vol.32Issue(7)：1123-1128,1191,7.DOI:10.3969/j.issn.1006-6233.2026.07.010
芹菜素通过调节NOS/cGMP/PKG信号通路改善慢性肾脏病大鼠的肾功能
Apigenin Improves Renal Function in Rats with Chronic Kidney Disease by Modulating the NOS/cGMP/PKG Signaling Pathway
摘要
Abstract
Objective:To explore the improving effect of apigenin on renal function in rats with chronic kidney disease(CKD)and its effects on nitric oxide synthase(NOS)/cyclic guanosine phosphate(cGMP)/protein kinase G(PKG)signaling pathway.Methods:Sixty male SD rats were randomly divided into control group,model group,positive drug valsartan,and low-,medium-,and high-dose apigenin groups,with 10 rats in each group.A 0.25%adenine solution at 150mg·kg-1·d-1 was intragastrically administered daily for 8 consecutive weeks to establish a CKD rat model.The weight of renal tissue in each group was measured.The levels of creatinine(SCr),urea nitrogen(BUN),uric acid(UA),IL-6,IL-1β and TNF-α in the se-rum as well as 24-hour urinary total protein(24-hour UTP)of rats were determined by automatic biochemical analyzer.HE staining and Masson staining were used to detect the pathological damage and fibrosis degree in renal tissues,respectively.Western blot was used to detect the expression of LC3,Beclin1,p62 and PKG proteins in renal tissues.Commercial assay kits were used to detect the levels of NOS,nitric oxide(NO),cGMP protein in renal tissues.Results:Compared with the control group,the renal tissue weight,SCr,BUN,UA,24-h UTP,p62,IL-6,IL-1β,and TNF-α of the rats were increased in the model group(P<0.05),while LC3Ⅱ/Ⅰ ratio,Beclin1,NOS,NO,cGMP,PKG were decreased(P<0.05),and renal tis-sues showed significant pathological damage and fibrotic lesions.Compared with the model group,the renal tissue weight,SCr,BUN,UA,24h UTP,p62,IL-6,IL-1β,and TNF-α of the rats in the positive drug group and low-,medium-,and high-dose apigenin groups were decreased(P<0.05),while LC3Ⅱ/Ⅰ rati-o,Beclin1,NOS,NO,cGMP,PKG were increased(P<0.05),the pathological damage and fibrosis degree of renal tissues were alleviated.Moreover,the effects of apigenin was dose-dependent(P<0.05).Conclu-sions:Apigenin can alleviate pathological damage and fibrosis of renal tissues by promoting autophagy and in-hibiting inflammatory responses,thereby improving renal function in rats with chronic kidney disease.Its mechanism may be related to the activation of the NOS/cGMP/PKG signaling pathway.关键词
慢性肾脏病/芹菜素/NOS/cGMP/PKG信号通路/自噬Key words
Chronic kidney disease/Apigenin/NOS/cGMP/PKG signaling pathway/Autoph-agy引用本文复制引用
赵笑晨,赵引,刘帅..芹菜素通过调节NOS/cGMP/PKG信号通路改善慢性肾脏病大鼠的肾功能[J].河北医学,2026,32(7):1123-1128,1191,7.基金项目
河北省医学科学研究课题计划资助(20241637) （20241637）
