郑州大学学报（医学版）2026，Vol.61Issue(4)：34-39,6.DOI:10.13705/j.issn.1671-6825.2026.02.098
藤黄酸调节IL-6/STAT3信号通路对急性胰腺炎大鼠肠道损伤的影响
Effects of gambogic acid on intestinal injury in rats with acute pancreati-tis by regulating IL-6/STAT3 signaling pathway
摘要
Abstract
Aim:To discuss the effects of gambogic acid on intestinal injury in rats with acute pancreatitis(AP)by regulating the IL-6/signal transducer and activator of transcription 3(STAT3)signaling pathway.Methods:Seventy-two SD rats were randomly divided into sham operation(sham)group,AP group,low-,medium-,and high-dose gambogic acid(L-,M-,H-gambogic acid)groups,and H-gambogic acid+STAT3 activator colivelin(H-gambogic acid+colivelin)group,with 12 rats in each group.The AP model was established by injection of sodium taurocholate into the pancreaticobiliary duct,while the sham group received an equal volume of normal saline.After modeling,the L-,M-,H-gambogic acid groups were administrated with 25,50,and 100 mg/kg gambogic acid by gavage,respectively,and the H-gambogic acid+colivelin group received 100 mg/kg gambogic acid by gavage and intraperitoneal injection of 1 mg/kg colivelin,once daily for 7 consecutive days.After the last administration,serum levels of amylase,lipase,and inflammatory factors(TNF-α,IL-6,IL-18,IL-1β)were detected by ELISA.Serum diamine oxidase(DAO)level was detected by colorimetric method.Pathological changes of pancreatic and colonic mucosal tissues were observed by HE staining.Apoptosis rate of colonic mucosal tissue,expression of zonula occludens-1(ZO-1)and IL-6/STAT3 signaling pathway-related proteins were detected by TUNEL staining and West-ern blot,respectively.Results:The AP group showed diffuse pancreatic acinar necrosis,capillary hemorrhage,inflammatory cell infiltration,colonic mucosal epithelial shedding,and inflammatory cell infiltration in the lamina propria and interepithe-lial spaces.The pancreatic and colonic mucosal damage alleviated in the L-,M-,and H-gambogic acid groups,whereas col-ivelin treatment reversed the effects of high-dose gambogic acid.Compared with the sham group,the AP group showed de-creased ZO-1 protein expression in colonic mucosal tissue,and increased serum levels of amylase,lipase,DAO,TNF-α,IL-6,IL-18,and IL-1β,as well as increased apoptosis rate,IL-6 protein expression,and p-STAT3/STAT3 in colonic mucosal tissue(P<0.05).Compared with the AP group,the L-,M-,and H-gambogic acid groups exhibited opposite trends in the a-bove indicators,and high dose had the best effects(P<0.05).Colivelin treatment reversed the effects of high-dose gambog-ic acid on these indicators except IL-6(P<0.05).Conclusion:Gambogic acid may alleviate intestinal injury in AP rats by inhibiting the IL-6/STAT3 signaling pathway.关键词
藤黄酸/IL-6/STAT3信号通路/急性胰腺炎/肠道损伤/大鼠Key words
gambogic acid/IL-6/STAT3 signaling pathway/acute pancreatitis/intestinal injury/rat分类
医药卫生引用本文复制引用
陈倩,张莉,刘颖,张丽贤..藤黄酸调节IL-6/STAT3信号通路对急性胰腺炎大鼠肠道损伤的影响[J].郑州大学学报（医学版）,2026,61(4):34-39,6.基金项目
衡水市市级科技计划自筹项目(2020014046Z) （2020014046Z）
