郑州大学学报（医学版）2026，Vol.61Issue(4)：40-44,5.DOI:10.13705/j.issn.1671-6825.2025.06.050
黄芩素调控NRG1/ErbB4通路对血管性痴呆大鼠认知功能及海马神经元的影响
Impacts of baicalein on cognitive function and hippocampal neuron in vascular dementia rats by adjusting NRG1/ErbB4 pathway
摘要
Abstract
Aim:To investigate the effects of baicalein on cognitive function and hippocampal neuron in rats with vas-cular dementia(VD)and to explore whether these effects are mediated through the regulation of neuregulin-1(NRG1)/erb-b2 receptor tyrosine kinase 4(ErbB4)pathway.Methods:Forty SD rats were established VD models and randomly assigned into 4 groups(n=10 each):VD group,low-dose baicalein group,high-dose baicalein group,and high-dose baicalein plus dacomitinib(an NRG1/ErbB4 pathway inhibitor,7.5 mg/kg)group.An additional sham operation group(n=10)was in-cluded.All treatments were administered via intragastric gavage.Neurological function was assessed using the Zea-Longa score and the Morris water maze test.Serum levels of TNF-α,IL-6,and IL-1β were measured by ELISA.Hippocampal histo-morphological changes and ultrastructural alterations were observed using HE staining and transmission electron microscopy,respectively.Malondialdehyde(MDA)content,glutathione peroxidase(GSH-PX)activity,and superoxide dismutase(SOD)activity in hippocampal tissues were determined via thiobarbituric acid method,colorimetric method,and hydroxylamine method,respectively.Protein expression levels of brain-derived neurotrophic factor(BDNF),Cleaved-caspase-3,myelin bas-ic protein(MBP),oligodendrocyte transcription factor1(Olig1),NRG1,and p-ErbB4/ErbB4 in the hippocampus were de-tected by Western blot.Results:Compared with the sham operation group,the VD group exhibited significantly enlarged in-ter-neuronal spaces,vacuolar degeneration,pyknotic nuclei,swollen and reduced mitochondria in hippocampal neurons,pro-longed escape latency,elevated serum levels of TNF-α,IL-1β,and IL-6,increased hippocampal MDA content and Cleaved-caspase-3 protein expression,alongside decreased target quadrant dwell time,number of platform crossings,hippocampal SOD and GSH-PX activities,and protein expression levels of BDNF,MBP,Olig1,NRG1,and p-ErbB4/ErbB4 ratio(all P<0.05).Compared with the VD group,both low-and high-dose baicalein groups showed significant improvements in hipp-ocampal tissue and neuronal morphology,as well as in all the above-mentioned parameters(P<0.05).Notably,co-adminis-tration of dacomitinib significantly attenuated the beneficial effects of baicalein on neurological dysfunction and neuronal in-jury in VD rats(P<0.05).Conclusion:Baicalein alleviates inflammation,oxidative stress,and neuronal injury,and im-proves neurological function in VD rats,potentially through the activation of the NRG1/ErbB4 pathway.关键词
黄芩素/NRG1/ErbB4通路/血管性痴呆/神经保护/大鼠Key words
baicalein/NRG1/ErbB4 pathway/vascular dementia/neuroprotection/rat分类
医药卫生引用本文复制引用
刘欢,肖骋..黄芩素调控NRG1/ErbB4通路对血管性痴呆大鼠认知功能及海马神经元的影响[J].郑州大学学报（医学版）,2026,61(4):40-44,5.基金项目
咸宁市市级科技计划项目(2020ZRKX03) （2020ZRKX03）
