郑州大学学报（医学版）2026，Vol.61Issue(4)：45-49,5.DOI:10.13705/j.issn.1671-6825.2025.05.190
松油烯-4-醇通过调控SIRT1/FOXO1信号通路对慢性肾病小鼠肾小管上皮-间质转化的影响
Effect of terpinen-4-ol on ameliorating renal tubular epithelial-mesenchy-mal transition via the SIRT1/FOXO1 signaling pathway in chronic kidney disease mice
摘要
Abstract
Aim:To investigate the effect of terpinen-4-ol on renal tubular epithelial-mesenchymal transition(EMT)in mice with chronic kidney disease(CKD)by regulating the sirtuin 1(SIRT1)/forkhead box O1(FOXO1)signaling pathway.Methods:A total of 24 mice were randomly divided into 4 groups:normal control group,CKD model group,and low and high dose terpinen-4-ol groups,with 6 mice in each group.Except the normal control group,CKD models were established in the other 3 groups.After successful modeling,the normal control group and CKD model group were given equal volumes of olive oil by gavage;low and high dose terpinen-4-ol groups were administered 10 and 20 mg/kg terpinen-4-ol by gavage,respectively,once a day for 6 consecutive weeks.After the experiment,blood samples were collected via retro-orbital puncture and serum BUN and Scr levels were measured;after euthanizing,kidney tissue was taken and observed for pathological changes using HE and Masson staining.Western blot was used to detect the expression levels of E-cadherin,α-SMA,Vimentin,SIRT1,and FOXO1 proteins.Another 36 mice were divided into 6 groups:Lv-NC group,Lv-NC+CKD group,Lv-NC+CKD+terpinen-4-ol group,Lv-Sirt1 RNAi group,Lv-Sirt1 RNAi+CKD group,Lv-Sirt1 RNAi+CKD+terpinen-4-ol group,with 6 mice in each group.The establishment of CKD model and the administration method of terpinen-4-ol(20 mg/kg)were the same as above.Lv-NC group,Lv-NC+CKD group,Lv-NC+CKD+terpinen-4-ol group were administered Lv-NC(5×107 U/mouse)via tail vein injection,while the other3 groups were injected Lv-Sirt1 RNAi(5×107 U/mouse)for2 consecutive weeks.After euth-anizing,kidney tissue was taken and Western blot was used to detect the expression levels of E-cadherin,α-SMA,Vimentin,SIRT1,and FOXO1 proteins.HE and Masson staining were used to observe the pathological changes in kidney tissue.Re-sults:Terpinen-4-ol could improve renal function damage in CKD mice,downregulate the expression levels of α-SMA and Vimentin proteins in renal tissue,and upregulate the expression levels of E-cadherin,SIRT1,and FOXO1 proteins(P<0.05).Knocking down Sirt1 expression can exacerbate kidney damage in CKD mice,further upregulate the expression levels of α-SMA and Vimentin proteins,downregulate the expression levels of E-cadherin,SIRT1,and FOXO1 proteins(P<0.05),while terpinen-4-ol could reverse this phenomenon(P<0.05).Conclusion:Terpinen-4-ol may affect EMT by acti-vating the SIRT1/FOXO1 signaling pathway,thereby improving kidney injury in CKD mice.关键词
松油烯-4-醇/肾小管上皮-间质转化/慢性肾病/沉默信息调节因子1/叉头盒蛋白O1/小鼠Key words
terpinen-4-ol/renal tubular epithelial-mesenchymal transition/chronic kidney disease/sirtuin 1/forkhead box O1/mouse分类
医药卫生引用本文复制引用
何丽,毕俊,王宇庭,肖红,何永祥,张彦燕..松油烯-4-醇通过调控SIRT1/FOXO1信号通路对慢性肾病小鼠肾小管上皮-间质转化的影响[J].郑州大学学报（医学版）,2026,61(4):45-49,5.基金项目
国家自然科学基金项目(82060791) （82060791）
贵州省中医药管理局中医药、民族医药科学技术研究项目(QZYY-2025-158) （QZYY-2025-158）
贵州省卫生健康委员会科学技术基金项目(gzwkj2025-543) （gzwkj2025-543）
