农业科技与技术（英文）2026，Vol.27Issue(1)：25-32,8.DOI:10.16175/j.cnki.1009-4229.2026.01.004
芥菜型油菜BjMADS23基因启动子克隆及表达载体构建
Cloning and Expression Vector Construction of BjMADS23 Promoter in Brassica juncea
摘要
Abstract
Cloning and analyzing the promoter of BjMADS23 is of great significance for understanding its involvement in regulating stress resistance in Brassica juncea.The promoter sequence of BjMADS23 was cloned via PCR,and cis-acting regulatory elements within this promoter were analyzed through the online tool PlantCARE.A plant expression vector pBjMADS23+GUS with fusion of the GUS gene was constructed by seamless cloning.The results showed that the cloned promoter sequence of BjMADS23 was 2 450 bp in length,harboring multiple cis-acting elements responsive to light,abscisic acid induction,anaerobic induction,salicylic acid induction,and drought induction.The plant expression vector pBjMADS23+GUS was successfully constructed,providing a foundation for subsequent studies on the transcriptional regulation of BjMADS23 in B.juncea.关键词
芥菜型油菜/BjMADS23基因/启动子/表达载体构建Key words
Brassica juncea/BjMADS23/Promoter/Expression vector construction引用本文复制引用
蒋湘云,袁玉辉,谢春凤,黄靖依,孙紫雨,钟品田,黄文津,王颖,刘显军..芥菜型油菜BjMADS23基因启动子克隆及表达载体构建[J].农业科技与技术（英文）,2026,27(1):25-32,8.基金项目
Supported by the National Undergraduate Training Program for Innovation and Entrepreneurship(S202410553021) （S202410553021）
Natural Science Foundation of Hunan Province(2025JJ70318).国家大学生创新创业项目专项(S202410553021) （2025JJ70318）
湖南省自然科学基金项目(2025JJ70318) （2025JJ70318）
