农业科技与技术（英文）2026，Vol.27Issue(1)：56-64,9.DOI:10.16175/j.cnki.1009-4229.2026.01.008
抗多花黄精根腐病生防菌A3-1的促生防病机理
Plant Growth-Promoting and Disease-Preventing Mechanisms of Biocontrol Strain A3-1 Against Root Rot of Polygonatum cyrtonema Hua
摘要
Abstract
This study aimed to explore the green biocontrol methods for root rot of Polygonatum cyrtonema Hua and alleviate the continuous cropping obstacles of P.cyrtonema in Xinhua County,Hunan Province.Four pathogenic fungal species isolated from diseased plants were taken as the target pathogens,and Bacillus subtilis A3-1 screened and identified in our laboratory was used as the biocontrol agent.The biocontrol potential of A3-1 against root rot of P.cyrtonema was systematically evaluated through plate confrontation experiments,evaluation of plant growth-promoting and fungal pathogen-inhibiting functions,antifungal activity evaluation of metabolites,and hyphal morphology observation.The results showed that A3-1 had multiple plant growth-promoting functions such as producing ammonia,fixing nitrogen,secreting siderophores,and solubilzing organic and inorganic phosphorus.Its ACC deaminase activity reached 65.04 U/mL,and it could secrete cellulase,protease,and amylase.The plate confrontation experiments showed that the strain exhibited the best inhibitory effect on Colletotrichum spaethianum,while its volatile metabolites had a high inhibition rate of 91.39%on Fusarium solani,while causing morphological distortion of hyphae.However,the sterile filtrate of this strain did not show significant antifungal activity.In summary,strain A3-1 showed dual functions of promoting plant growth and inhibiting fungal pathogens,with its volatile gas as the key antifungal component,demonstrating promising application prospects in biocontrol.关键词
多花黄精/根腐病/生物防治/连作障碍/促生功能Key words
Polygonatum cyrtonema Hua/Root rot/Biocontrol/Continuous cropping obstacles/Plant growth-promoting function引用本文复制引用
贺梅,彭逸群,姚璇,黄海,朱倩霞,杨淞,陈勇,郭开发..抗多花黄精根腐病生防菌A3-1的促生防病机理[J].农业科技与技术（英文）,2026,27(1):56-64,9.基金项目
Supported by the Project of Department of Science and Technology of Hunan Province(2023JJ50466,2024JJ7279) （2023JJ50466,2024JJ7279）
Postgraduate Scientific Research Innovation Project of Hunan Province(LXBZZ2024383).湖南省科技厅项目(2023JJ50466,2024JJ7279) （LXBZZ2024383）
湖南省研究生科研创新项目(LXBZZ2024383) （LXBZZ2024383）
