中医药导报2026，Vol.32Issue(7)：32-35,41,5.DOI:10.13862/j.cn43-1446/r.2026.07.005
石榴皮多酚软膏调节GATA6及相关因子抗痤疮机制
Anti-Acne Mechanism of Pomegranate Peel Polyphenols Ointment via Regulation of GATA6 and Related Factors
摘要
Abstract
Objective:To investigate the anti-acne mechanism of pomegranate peel polyphenols ointment by observing their effects on sebaceous gland spots and serum levels of transcription factor GATA6,transforming growth factor-β(TGF-β),and interleukin-10(IL-10)in golden hamsters.Methods:Totally 36 golden hamsters were randomly divided into six groups[blank group,vehicle group(1 g ointment base),low-dose group(0.480％pomegranate peel polyphenols ointment),medium-dose group(0.960％pomegranate peel polyphenols ointment),high-dose group(1.920％pomegranate peel polyphenols ointment),and positive control group(0.025％Isotretinol yogurt cream)].After 4 weeks of treatment,therapeutic effects were evaluated.Histopathological changes in sebaceous glands were assessed by Hematoxylin-Eosin(HE)staining.Serum IL-10,TGF-β,and TGF-β2 were quantified by enzyme linked immunosorbent assay(ELISA),and cutaneous GATA6 expression was measured by Western blotting.Results:HE staining results showed that pomegranate peel polyphenols ointment reduced sebaceous gland volume,glandular lobes and mature sebocytes in a dose-dependent manner,and the efficacy of high-dose group was similar to positive group.The vehicle group showed lower IL-10 level than blank group,while higher TGF-β1 and TGF-β2 level than blank group(P＜0.05).All treatment groups reversed the above abnormal indexes,increased IL-10 and decreased TGF-β1 and TGF-β2(P＜0.05),with obvious dose-dependent effect.The high-dose group presented the best therapeutic effect,while low-dose group and medium-dose group showed mild efficacy without significant inter-group difference(P＞0.05).The GATA6 expression in vehicle group was lower than that of blank group(P＜0.05).All treatment groups significantly up-regulated GATA6 expression(P＜0.05),and there was no statistical difference between high-dose group and positive control group(P＞0.05).Con-clusion:Pomegranate peel polyphenols ointment may exert its anti-acne effects by regulating GATA6 and its related factors,thereby influencing the secretion of inflammatory cytokines,preventing abnormal keratinization,and so on.关键词
痤疮/石榴皮多酚/GATA6/皮脂腺斑/金黄地鼠Key words
acne/pomegranate peel polyphenols/GATA6/sebaceous gland spots/golden hamster分类
医药卫生引用本文复制引用
杨逸璇,吴淑辉,赵丹,黄慧源,李鹏宇,朱明芳..石榴皮多酚软膏调节GATA6及相关因子抗痤疮机制[J].中医药导报,2026,32(7):32-35,41,5.基金项目
长沙市自然科学基金一般项目(kq2502264) （kq2502264）
湖南中医药大学科研基金项目(2021XJJJ060) （2021XJJJ060）
