摘要
Abstract
Objective:To investigate the protective effect and underlying molecular mechanism of curcumin against ethanol-induced acute gastric mucosal injury.Methods:Fifty SPF-grade Kunming mice were randomly assigned to five groups:blank control group(NC),model group(MD),low-dose curcumin group(Curcumin-L,50 mg/kg),medium-dose curcumin group(Curcumin-M,100mg/kg),and high-dose curcumin group(Curcumin-H,200 mg/kg),with 10 mice per group.An animal model of ethanol-triggered acute gastric mucosal injury was constructed,and corresponding interventions were implemented.Hematoxylin-eosin(HE)staining was performed to observe histopathological lesions in gastric mucosal tissues.Enzyme-linked immunosorbent assay(ELISA)was adopted to measure serum levels of gastrointestinal hormones,inflammatory mediators and oxidative stress markers.Immunohistochemistry was used to detect proliferating cell nuclear antigen(PCNA)expression.Western blotting was conducted to quantify the protein abundances of SIRT1,Nrf2 and HO-1 in gastric mucosa.Results:Curcumin markedly lowered the injury incidence and gastric mucosal lesion score in mice(P＜0.01),elevated serum Ghrelin concentrations(P＜0.05),and downregulated serum MTL and 5-HT levels(P＜0.05).It also decreased the contents of pro-inflammatory cytokines(IL-6,IL-1 β,TNF-α)and malondialdehyde(MDA)(P＜0.05),while enhancing the activities of superoxide dismutase(SOD)and glutathione peroxidase(GSH-Px),together with PCNA expression(P＜0.05).Moreover,curcumin markedly upregulated the protein expression of SIRT1,Nrf2 and HO-1 within gastric mucosal tissues(P＜0.05).Conclusion:Curcumin ameliorates ethanol-induced acute gastric mucosal injury through activation of the SIRT1/Nrf2/HO-1 signaling pathway.关键词
急性胃黏膜损伤/姜黄素/SIRT1/Nrf2/HO-1通路/小鼠Key words
acute gastric mucosal injury/curcumin/SIRT1/Nrf2/HO-1 signaling pathway/mice分类
医药卫生
