中医药导报2026，Vol.32Issue(7)：59-64,109,7.DOI:10.13862/j.cn43-1446/r.2026.07.010
特征图谱联合薄层色谱鉴别拳参标准汤剂、配方颗粒及易混品
Identification of Standard Decoction,Formula Granules and Adulterants of Quanshen(Polygonum Bistorta L.)Based on Characteristic Chromatogram and Thin Layer Chromatography
王冬月 1高扬 1张志强1
作者信息
- 1. 北京康仁堂药业有限公司,北京 101301||中药配方颗粒关键技术国家地方联合工程研究中心,北京 101301||北京市中药配方颗粒工程技术研究中心,北京 101301
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摘要
Abstract
Objective:To establish methods for thin-layer chromatography(TLC)and quantitative characteristic chromatogram to provide a scientific basis for the identification and quality control of Quanshen(Polygonum Bistorta L)standard decoction and its adulterants.Methods:TLC was employed to identify the Quanshen(Polygonum Bistorta L.)standard decoction,formula granules,and its adulterants,using Quanshen(Polygonum Bistorta L.)reference herbs,gallic acid and chlorogenic acid as controls.Additionally,ultra-performance liquid chromatog-raphy(UPLC)was used to establish a quantitative characteristic chromatogram of Quanshen(Polygonum Bistorta L.).The characteristic chromatograms were analyzed using similarity evaluation software,cluster analysis(CA),principal component analysis(PCA)and orthogonal partial least square discriminant analysis(OPLS-DA)to identify the main differences between Quanshen(Polygonum Bistorta L.)and its adulterants.The differential components were quantitatively determined.Results:TLC revealed distinct fluorescence spots between the standard decoction of Quanshen(Polygonum Bistorta L)and its adulterants.The similarity evaluation software analyzed the characteristic chromatograms of 15 batches of Quanshen(Polygonum Bistorta L.)standard decoction,establishing a reference chromatogram.The similarity of Quanshen(Polygonum Bistorta L.)samples was greater than 0.90,while the similarity of adulterants(such as Polygonum viviparum L.and Polygonum paleaceum Wall.)was less than 0.90.CA,PCA and PLS-DA identified gallic acid and chlorogenic acid as the primary differential components between Quanshen(Polygonum Bistorta L.)and its adulterants.Quantitative results showed significant differences in the content of these two components.Conclusion:The established TLC and liquid chromatography methods can quickly,accurately and objectively identify Quanshen(Polygonum Bistorta L.)and its adulterants,significantly improving the accuracy of clinical drug use.关键词
拳参/混伪品/标准汤剂/配方颗粒/特征图谱/薄层色谱/含量测定Key words
Quanshen(Polygonum Bistorta L.)/contaminated product/standard decoction/formula granules/characteristic profile/thin-layer chromatography/content determination分类
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王冬月,高扬,张志强..特征图谱联合薄层色谱鉴别拳参标准汤剂、配方颗粒及易混品[J].中医药导报,2026,32(7):59-64,109,7.
