家畜生态学报2026，Vol.47Issue(8)：41-49,9.DOI:10.3969/j.issn.1673-1182.2026.08.006
牛至精油对羔羊盲肠组织形态、菌群结构及功能的影响
Effect of Oregano Essential Oil on Cecal Tissue Morphology,Microbial Community Structure and Function in Lambs
摘要
Abstract
This experiment aimed to screen the optimal supplementation level of oregano essential oil(OEO)for Saanen goat kids.Forty-eight male Saanen goat kids with similar birth weights were selected and randomly assigned to three treatment groups(4 replicates per group,4 kids per replicate).At 7 days of age,the kids were fed starter diets supplemented with 0 g/d(CON),1 g/d(OEO1),and 2 g/d(OEO2)OEO.At 70 days of age,the kids were weighed in the morning after fasting,and 6 kids from each group were randomly slaughtered to collect cecal tissue and contents for analysis.The results showed that OEO had no significant effects on cecal pH or tissue morphology(P＞0.05).A total of 1,522,540 high-quality reads and 5,157 ASVs were obtained from the 18 cecal content samples.The chao1,shannon,simpson,observed_features,and pielou_e indices in the OEO2 group were extremely higher than those in the OEO1 and CON groups(P＜0.01),while the dominance index in the CON group was significantly higher than that in the OEO1 and OEO2 groups(P＜0.01).LEfSe analysis revealed that the OEO1 group was primarily characterized by Oscillospiraceae within the order Oscillospirales.The OEO2 group showed significant enrichment of Christensenellaceae_R-7_group and Monoglobus(phylum Bacillota),as well as Bacteroides,Prevotellaceae UCG-004,and Rikenellaceae_RC9_gut_group(phylum Bacteroidota).The CON group showed significant enrichment of Agathobacter,Coprococcus,and Ruminococcus(phylum Ba-cillota).Tax4Fun functional prediction analysis indicated that the cecal microbiota were primarily involved in carbohydrate metabolism,membrane transport,replication and repair,amino acid metabolism,transla-tion,energy metabolism,nucleotide metabolism,signal transduction,cell motility,and metabolism of co-factors and vitamins.Correlation analysis showed that pH was positively correlated with Agathobacter and unclassified_Clostridia_UCG.014(P＜0.05),and negatively correlated with Christensenellaceae_R.7_group,unclassified_UCG.010,and Rikenellaceae_RC9_gut_group(P＜0.05).Relative cecal volume was negatively correlated with Bacteroides(P＜0.05),and mucosal layer thickness was negatively correla-ted with Bacteroides,unclassified_Eubacterium_coprostanoligenes_group,and Monoglobus(P＜0.05).In conclusion,the use of OEO in goat kid production can effectively improve the structure and function of the cecal microbiota,and the optimal dietary supplementation level of oregano essential oil is 2 g/d.关键词
OEO/羔羊/盲肠/16SrDNAKey words
Oregano Essential Oil/lambs/cecum/16SrDNA分类
农业科技引用本文复制引用
李云让,靳继鹏,孟泉禄,边会龙,赵东林,沈丽,李拴军,闫恒周..牛至精油对羔羊盲肠组织形态、菌群结构及功能的影响[J].家畜生态学报,2026,47(8):41-49,9.基金项目
2024年度陕西省教育厅一般专项科学研究计划项目(24JK0303) （24JK0303）
宝鸡职业技术学院2024年度院级重点课题(2024053Z) （2024053Z）
