江苏大学学报（医学版）2026，Vol.36Issue(4)：305-310,6.DOI:10.13312/j.issn.1671-7783.y250041
雷公藤甲素对小鼠血睾屏障的损伤作用及机制
The damaging effect and mechanism of triptolide on the blood-testis barrier in mice
摘要
Abstract
Objective:To investigate the damaging effects of triptolide on the blood-testis barrier and its underlying molecular mechanisms.Methods:Nine healthy male C57BL/6 mice(20-25 g)were randomly divided into control group,low-dose triptolide group(0.2 mg/kg),and high-dose triptolide group(0.5 mg/kg),with 3 animals per group.After continuous administration for 28 days,body weight,testicular volume,and testicular histomorphology were observed in mice exposed to different doses of triptolide.Intergroup comparisons of testis-to-body weight ratio,germ cell apoptosis level,expression of blood-testis barrier-related proteins and barrier leakage were performed among the three groups.Results:Compared with the control group,mice in triptolide-treated groups exhibited markedly reduced body weight gain,testicular weight and testis-to-body weight ratio(all P＜0.01).HE staining revealed massive loss of germ cells and prominent vacuolization in the seminiferous tubular lumen of triptolide-exposed mice.The sperm count in epididymal tissues was decreased upon triptolide administration,and this reduction was further aggravated in the high-dose group,accompanied by abundant necrotic germ cells.TUNEL assay revealed markedly elevated apoptosis in seminiferous tubules of triptolide-treated mice(all P＜0.01).Western blotting indicated that the expression levels of blood-testis barrier-related proteins(E-cadherin,occludin,β-catenin,VE-cadherin,Arp3,and Claudin 11)were significantly downregulated in triptolide-treated groups(all P＜0.01).Biotin tracer staining demonstrated varying degrees of blood-testis barrier injury in triptolide-treated mice,and the severity of barrier leakage increased in a dose-dependent manner.Conclusion:Triptolide treatment can be used to stably establish a mouse model of blood-testis barrier injury.Its mechanism is closely related to the synergistic regulation of multiple pathways,including Sertoli cell apoptosis,downregulated expression of blood-testis barrier-related proteins and oxidative stress response.关键词
血睾屏障/雷公藤甲素/生物素/细胞凋亡Key words
blood-testis barrier/triptolide/biotin/apoptosis分类
医药卫生引用本文复制引用
王宏亮,王铸,张建文,赖裕林,邓琼..雷公藤甲素对小鼠血睾屏障的损伤作用及机制[J].江苏大学学报（医学版）,2026,36(4):305-310,6.基金项目
深圳市基础研究专项/自然科学基金资助项目(JCYJ20240813114409013) （JCYJ20240813114409013）
深圳市龙华区医学会医学科研专项课题(2023LHMA08) （2023LHMA08）
