兰州大学学报（医学版）2026，Vol.52Issue(5)：8-14,7.DOI:10.13885/j.issn.2097-681X.M20250557
葡萄籽原花青素对过氧化氢氧化损伤兔晶状体的保护作用
Protective effects of grape seed proanthocyanidins on rabbit lenses subjected to oxidative damage induced by hydrogen peroxide
摘要
Abstract
Objective To investigate the protective effects and molecular mechanisms of grape seed proan-thocyanidins against hydrogen peroxide-induced oxidative damage in rabbit lens and its lens epithelial cells.Methods Healthy rabbit lenses were randomized into five groups:normal control,model control(H2O2-dam-aged),low-mass concentration grape seed proanthocyanidin(25 μg/mL),medium-mass concentration(50 μg/mL),and high-mass concentration(100 μ g/mL).After 24 h culture,lens opacity and histopathology were assessed.Enzymatic activities(total superoxide dismutase,catalase,glutathione peroxidase)and malondialde-hyde levels were quantified using commercial kits.Immunohistochemistry evaluated BCL-2 and BAX protein expression.Results Lens opacity rates were 100％(model control)versus 10％(normal control),with inter-vention groups showing 80％(low),50％(medium),and 30％(high),respectively.Model control exhibited severe epithelial damage(cell detachment,disordered arrangement,irregular morphology,fiber breakage),while all treatments alleviated these changes.Antioxidant capacity was significantly decreased in model control(reduced enzyme activities,elevated malondialdehyde;all P<0.05 vs normal),partially reversed by medium/high-mass concentration interventions.Normal lenses showed higher BCL-2 than BAX protein expression,whereas model control demonstrated suppressed BCL-2,upregulated BAX protein,and decreased BCL-2/BAX protein ratio(P<0.01).All grape seed proanthocyanidin groups normalized protein expression and elevated the ratio.Conclusion Grape seed proanthocyanidins protect rabbit lenses and lens epithelial cells against H2O2-induced damage by enhancing antioxidant defenses and regulating apoptosis-related proteins BCL-2 and BAX expression.关键词
葡萄籽原花青素/氧化损伤/晶状体上皮细胞/白内障/细胞凋亡/BCL-2/BAX比值Key words
grape seed proanthocyanidin/oxidative damage/lens epithelial cell/cataract/apoptosis/ratio of BCL-2 and BAX分类
医药卫生引用本文复制引用
李增佑,孙永红,闵光宁,张小郁..葡萄籽原花青素对过氧化氢氧化损伤兔晶状体的保护作用[J].兰州大学学报（医学版）,2026,52(5):8-14,7.基金项目
甘肃省药品监督管理局药品科学研究项目资助(2022GSMPA007,2022GSMPA006,2024GSMPA041) （2022GSMPA007,2022GSMPA006,2024GSMPA041）
